HPLC法测定原料乳中庆大霉素残留的研究  

Determination of Gentamicin in Raw Milk by High-performance Liquid Chromatopraphy

作　　者：赵静[1,2] 刘婷[1] 蔡柏岩[1] 刘鹏 钱镭[1] 

机构地区：[1]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150066 [2]国家乳业工程技术研究中心,黑龙江哈尔滨150086

出　　处：《食品研究与开发》2015年第9期94-97,共4页Food Research and Development

基　　金：黑龙江省高等学校青年学术骨干支持计划(项目编号:1253G048)

摘　　要：建立测定原料奶中庆大霉素C组分的高效液相色谱法。通过加入适量沉淀剂除去样品中大部分蛋白质,采用邻苯二甲醛试剂柱前衍生化,利用C18色谱柱,以含有0.02 mol/L的甲醇-醋酸溶液为流动相进行梯度洗脱,用荧光检测器检测,激发波长340 nm、发射波长450 nm。结果表明,回收率在85.6%~97.3%,精密度（RSD）为1.21%~4.04%,最低检出限为0.2μg/m L。该方法可用于原料奶中庆大霉素残留4种组分的测定。To establish a HPLC method for determination of gentamicin in the raw milk. Protein in milk were mostly eliminated by the precipitators, OPA was used for derivatization, and then separated on C 18 column with a mixed solution of methanol-acetic acid as mobile phase, and excitation and emission wavelength are 340 nm and 450 nm with a fluorescence detector The recovery were 85.6 %-97.3 % ,the relative standard deviations （RSD） were 1.21%-4.04 %, the minimum detectable values were 0.2μg/mL. This method can be used for the routine analysis of gentamicin in raw milk.

关 键 词：高效液相色谱 庆大霉素 邻苯二甲醛 柱前衍生 

分 类 号：O657.72[理学—分析化学] TS252.7[理学—化学]