5种中药颗粒剂与水煎剂对5种耐药菌株的体外抑菌作用比较  

Comparison on in vitro antibacterial activities for five kinds of trditional Chinese medicine granules and decoctions against five kinds of clinical drug-resistant strains

作　　者：李蔼文[1] 谭俊青[1] 王康椿 黄双旺[1] 周兵[1] 刘启波[1] 

机构地区：[1]广东省第二中医院检验科,广东广州510095

出　　处：《检验医学》2015年第6期567-570,共4页Laboratory Medicine

摘　　要：目的比较5种中药(黄芩、连翘、薄荷、蒲公英、苦参)颗粒剂与水煎剂对5种临床分离耐药菌株的体外抑菌效果。方法根据中药抗耐药菌的相关研究,选取5种有抑菌效果的中药颗粒剂与水煎剂,取血平板分离的5种临床耐药菌株[包括耐甲氧西林金黄色葡萄球菌(MRSA)、铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌及鲍曼不动杆菌],进行常规中药药敏纸片琼脂扩散法,测定抑菌圈直径,比较水煎剂和颗粒剂的抑菌效果。结果5种中药对5种耐药菌的抑菌强度不同,黄芩抑菌效果最好。同一种中药颗粒剂与水煎剂相比:对于MRSA,黄芩、连翘、薄荷、蒲公英、苦参2种剂型均有抑菌效果,差异无统计学意义(P>0.05);对于耐药性铜绿假单胞菌、鲍曼不动杆菌,黄芩、连翘、薄荷2种剂型均有抑菌效果,差异无统计学意义(P>0.05);对于大肠埃希菌、肺炎克雷伯杆菌,黄芩的2种剂型均有抑菌效果,差异无统计学意义(P>0.05)。结论同等浓度的黄芩、连翘、薄荷、蒲公英、苦参,颗粒剂和水煎剂抑菌效果无明显差异。Objective To compare in vitro antibacterial activities for 5 kinds of traditional Chinese medicine granules and decoctions（ Baicalin,Forsythia,Mint,Dandelion and Sophora） against 5 kinds of clinical drug-resistant strains. Methods According to the related researches on traditional Chinese medicine against resistant bacteria,5kinds of traditional Chinese medicine granules and decoctions were selected,and 5 kinds of clinical drug-resistant strains from blood plate were collected. They included meticillin-resistant Staphylococcus aureus（ MRSA）,Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumoniae and Acinetobacter baumannii. The disc agar diffusion method was used,and the diameter of bacteriostatic ring was measured. The bacteriostatic effects were analyzed statistically. Results Five traditional Chinese medicines showed different antibacterial activities,with Baicalin being the most potent. The same kind of traditional Chinese medicine granule and decoction was compared. For MRSA,Baicalin,Forsythia,Mint,Dandelion and Sophora had antibacterial effects,and there was no statistical significance（ P〉0. 05）. For Pseudomonas aeruginosa and Acinetobacter baumannii,Baicalin,Forsythia and Mint had antibacterial effects,and there was no statistical significance（ P〉0. 05）. For Escherichia coli and Klebsiella pneumoniae,Baicalin had antibacterial effects,and there was no statistical significance（ P〉0. 05）. Conclusions The same concentrations of Baicalin,Forsythia,Mint,Dandelion and Sophora decoctions have not obvious difference for antibacterial effect.

关 键 词：中药颗粒剂 中药水煎剂 抑菌圈 临床耐药菌株 

分 类 号：R446.15[医药卫生—诊断学]