一种肺炎链球菌常见血清型多重PCR分型方案的评估  

Evaluation on multiplex PCR for detecting the common serotypes of one kind of Streptococcus pneumoniae

作　　者：潘芬[1] 赵声远[2] 秦惠宏[1] 祝俊英 肖淑珍[2] 张泓[1] 

机构地区：[1]上海交通大学附属儿童医院检验科,上海200040 [2]上海交通大学医学院附属瑞金医院临床微生物科,上海200025

出　　处：《检验医学》2015年第6期599-602,共4页Laboratory Medicine

摘　　要：目的评估一种肺炎链球菌常见血清型多重聚合酶链反应(PCR)分型方案的分型能力。方法以传统荚膜肿胀试验为参考,采用多重PCR分型方案对50株肺炎链球菌进行血清分型。结果 50株肺炎链球菌经传统荚膜肿胀试验分为19F型9株、19A型9株、23F型9株、6A/B型9株、14型9株和15B/C型5株;而多重PCR分型方案结果为19F型8株、19A型10株、23F型9株、6A/B型9株、14型10株和15B/C型4株,2种方法符合率为96%,Kappa值为0.952。结论多重PCR分型技术与常规方法一致性好,具有简便、快速、成本相对较低等优点,能用于监测临床肺炎链球菌流行血清型。Objective To evaluate the performance of multiplex polymerase chain reaction（ PCR） for detecting the common serotypes of one kind of Streptococcus pneumoniae. Methods A total of 50 isolates of Streptococcus pneumoniae were analyzed by multiplex PCR with traditional quellung reaction as reference method for detecting the common serotypes of Streptococcus pneumoniae. Results The 50 isolates were typed by traditional quellung reaction with 9isolates being 19 F,19A,23 F,6A / B and 14,respectively,and 5 isolates being 15 B / C. However,according to the results of multiplex PCR,8 of 50 isolates were serotype 19 F,and there also were 19A（10 isolates）,23F（9 isolates）,6A / B（9 isolates）,14（ 10 isolates） and 15 B / C（ 4 isolates）,which showed coincidence rate 96%. The value of Kappa was 0. 952. Conclusions Multiplex PCR has good consistency with traditional method,and it has the advantages of easy, rapid performance and low costing, which could provide the reference for monitoring the serotypes of Streptococcus pneumoniae in clinic.

关 键 词：多重聚合酶链反应 血清型 肺炎链球菌 

分 类 号：Q503[生物学—生物化学]