铜绿假单胞菌16S rRNA甲基化酶基因和氨基糖苷修饰酶基因的研究  

Studies of 16S rRNA methylase gene and aminoglycoside modifying enzyme gene in Pseudomonas aeruginosa

作　　者：彭敬红[1] 侯彦强[1] 谢多双[2] 刘军[1] 郑蓉[3] 

机构地区：[1]上海交通大学附属第一人民医院松江分院检验科,上海201600 [2]湖北医药学院附属太和医院院感科,湖北十堰442000 [3]湖北医药学院附属太和医院妇科,湖北十堰442000

出　　处：《检验医学》2015年第6期613-616,共4页Laboratory Medicine

基　　金：湖北省教育厅重点课题资助项目(D20112106);上海市松江区科学技术攻关项目(13SJGGYY17)

摘　　要：目的了解氨基糖苷类抗菌药物耐药的铜绿假单胞菌16S r RNA甲基化酶基因和氨基糖苷修饰酶基因的携带情况,探讨铜绿假单胞菌对氨基糖苷类药物的耐药机制。方法收集临床分离的氨基糖苷类抗菌药物耐药的铜绿假单胞菌35株,采用VITEK 2 Compact全自动微生物分析系统进行鉴定及体外药物敏感性试验,聚合酶链反应(PCR)检测arm-A、rmt-A、rmt-B、rmt-C、rmt-D 5种16S r RNA甲基化酶基因和aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰb、aac(6')-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ6种氨基糖苷修饰酶基因。结果对氨基糖苷类药物耐药的铜绿假单胞菌同时对多种其他抗菌药物耐药,仅碳青霉烯类的亚胺培南耐药率较低,为5.7%。21株检出rmt-B 16S r RNA甲基化酶基因,占60.0%;30株检出氨基糖苷修饰酶基因,占85.7%,其中28株检出aac(3)-Ⅱ基因,占80.0%,18株检出ant(3″)-Ⅰ基因,占51.4%,其余8种基因未检出。结论在铜绿假单胞菌对氨基糖苷类抗菌药物耐药的菌株中,检测出rmt-B 16S r RNA甲基化酶基因和aac(3)-Ⅱ、ant(3″)-Ⅰ氨基糖苷修饰酶基因,铜绿假单胞菌多重耐药现象严重。Objective To investigate 16 S rRNA methylase gene and aminoglycoside modifying enzyme gene in aminoglycoside-resistant clinical isolates of Pseudomonas aeruginosa,and to investigate the mechanism of drug resistance to aminoglycosides. Methods A total of 35 isolates of aminoglycoside-resistant clinical isolates of Pseudomonas aeruginosa were collected,and the identification and susceptibility testing in vitro were performed by VITEK 2 Compact automatic microbial analysis system. Five kinds of 16 S rRNA methylase genes（ arm-A,rmt-A,rmt-B,rmt-C and rmt-D）and 6 kinds of aminoglycoside modifying enzyme genes [aac（ 3）-Ⅰ,aac（ 3）-Ⅱ,aac（ 6＇）-Ⅰb,aac（ 6＇）-Ⅱ,ant（3″）-Ⅰand ant（2″）-Ⅰ] were detected by polymerase chain reaction（ PCR）. Results Aminoglycoside-resistant Pseudomonas aeruginosa showed being multi-drug resistant to a variety of antibiotics. Only the resistance rate to imipenem was low（ 5. 7%）. There were 21 isolates of Pseudomonas aeruginosa carried rmt-B 16 S rRNA methylase gene,accounting for 60. 0%,and 30 isolates of Pseudomonas aeruginosa carried aminoglycoside modifying enzyme genes,accounting for 85. 7%,among them,28 isolates carried aac（ 3）-Ⅱ aminoglycoside modifying enzyme genes,accounting for 80. 0%,and 18 isolates carried ant（ 3″）-Ⅰ aminoglycoside modifying enzyme genes,accounting for51. 4%. The other 8 isolates were not detected any genes. Conclusions Aminoglycoside-resistant isolates of Pseudomonas aeruginosa carry rmt-B 16 S rRNA methylase gene and aac（ 3）-Ⅱ and ant（ 3″）-Ⅰ aminoglycoside modifying enzyme genes. Furthermore,the multi-drug resistances of Pseudomonas aeruginosa are serious.

关 键 词：铜绿假单胞菌 16S rRNA甲基化酶基因 氨基糖苷修饰酶基因 耐药性 

分 类 号：R378.99[医药卫生—病原生物学]