精子存活实验预测人工授精后临床妊娠的价值  

Value of Sperm Survival Experiment in Predicting Clinical Pregnancy After Artificial Insemination

作　　者：吴成勇[1] 杨满[1] 林梅[1] 蔡敏生[1] 

机构地区：[1]广东省梅州市人民医院生殖中心,广东梅州514031

出　　处：《临床医学工程》2015年第6期718-720,共3页Clinical Medicine & Engineering

摘　　要：目的研究精子存活实验对夫精人工授精(IUI)后临床妊娠的预测价值。方法选择2012年1月至2014年6月在我院行IUI治疗的不孕夫妇125对,根据妊娠与否分为妊娠组(26例)和非妊娠组(99例),对精子进行48小时培养,对比两组精子体外存活情况。结果两组精液优化处理前精子总数、精子形态和前向运动精子比例比较差异不显著(P>0.05);精液优化处理24小时和48小时后,妊娠组前向运动精子比例明显高于未妊娠组,差异有统计学意义(P<0.01)。精液培养24时和48时ROC曲线下面积分别为96.2%和93.9%,最佳cut off截断值分别为45.4%和31.8%。结论人工授精患者进行体外培养精子存活实验,是预测人工授精成功与否的一个可靠指标,值得临床应用。Objective To study the value of sperm survival experiment in predicting clinical pregnancy after intrauterine insemination（IUI） with husband＇s sperm. Methods 125 infertile couples treated in our hospital from January 2012 to June 2014 were selected and divided into pregnant group（26 cases） and non-pregnancy group（99 cases）, all couples were given IUI for treating. The sperms were cultured for 48 hours, and the survival conditions of sperm in vitro between two groups were compared. Results Before the optimizing process of semen,the sperm count, percentage of sperm morphology and progressive sperm in two groups had no statistical difference（P〉0.05）. After 24 hours and 48 hours of the semen optimizing process, the percentage of progressive sperm in pregnant group was higher than that of non-pregnancy group, with statistical difference（P〈0.01）. The area under ROC curve at 24 hours and 48 hours of the semen culture were 96.2% and 93.9%respectively, the best cut off value were 45.4% and 31.8% respectively. Conclusions For patients with IUI, sperm survival experiment is a reliability index for predicting the success of pregnancy, which is worthy of clinical application.

关 键 词：精子存活实验 夫精人工授精 前向运动精子 妊娠 

分 类 号：R713.7[医药卫生—妇产科学]