热处理对经历高温锻炼的玉米象保护酶活性的影响  

EFFECT OF HEAT TREATMENT ON THE SPECIFIC ACTIVITIES OF PROTECTIVE ENZYMES IN SITOPHILUS ZEAMAIS MOTSCHULSKY WITH HIGH TEMPERATURE HARDENING

作　　者：张会娜[1] 吕建华[1] 张晨光[1] 白旭光[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2015年第3期76-80,共5页Journal of Henan University of Technology：Natural Science Edition

基　　金："十二五"国家高技术研究发展计划(863计划)项目(2012AA101705-2);河南省教育厅科技攻关项目(12A210003);河南工业大学基础研究重点培育基金项目(11JCYJ01);郑州市科技发展计划项目(2001NYXM228)

摘　　要：为明确热处理对经历过短时高温锻炼的玉米象(Sitophilus zeamais Motschulsky)成虫保护酶活性的影响,先将玉米象成虫置于36℃环境中锻炼5 h,然后放在43、47、51、57℃高温下处理不同时间,测定试虫体内超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Peroxidase,POD)和过氧化氢酶(Catalase,CAT)的活性变化.结果表明,随着热处理时间的延长,玉米象体内SOD活性先升高后降低,CAT活性则是先降低后升高,POD活性变化则因温度不同而异.本研究结果对理解昆虫的耐热机制、制定科学高效的热处理杀虫策略具有重要理论意义.The maize weevil, Sitophilus zeamais Mostchulsky （Coleoptera： Curculionidae）, ranks as one of the most destructive insect pests of stored cereal grains in the world. Conventional control of stored-grain insect pests, particularly using phosphine fumigation, has resulted in serious challenges, such as insecticide resistance, unacceptable pesticide residues in food and environmental contamination. As an efficient and environment-friendly alternative to synthetic pesticides, heat treatment has been focused on controlling insect pests for recent years. However, little is known about the effects of heat treatment on the specific activities of protective enzymes in stored grain insects. In order to explore the effects of heat treatment on protective enzymes＇ specific activities in S. zeamds with short-period high temperature hardening, S. zeamais adults were firstly hardened at 36℃ for 5 h, then treated at high temperatures of 43, 47, 51 and 57 ℃ for different time. Subsequently, the specific activities of three protective enzymes, superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） in S. zeamais adults, were investigated in this paper. The results showed that the specific activities of SOD in S. zeamais adults firstly increased, then decreased, and the specific activities of CAT firstly decreased, then increased with the increasing heat treatment time, while the specific activities of POD varied during the heat treatment. Free radicals will accumulate in insects when they suffer in heat stress conditions, and this will result in oxidative damage to biological function molecules of insects. The specific activities of protective enzymes represent the insect＇ s abilities to eliminate free radicals and adaptation to stress environment. These results will be in favor of understanding the heat tolerance mechanism of insects in heat stress environment and providing guidance to design the scientific and effective heat disinfestation strategy.

关 键 词：高温锻炼 玉米象 SOD POD CAT 

分 类 号：TS210[轻工技术与工程—粮食、油脂及植物蛋白工程]