桃儿七地下茎内生真菌的分离和鉴定  

Isolation and Identification of Endophytic Fungi Obtained from Underground Stems of Sinopodophyllum hexandrum

作　　者：祝英[1,2] 王治业[1] 杨晖[1] 周剑平[1] 

机构地区：[1]甘肃省科学院生物研究所,甘肃兰州730000 [2]兰州大学草地农业生态系统国家重点实验室生命科学学院,甘肃兰州730000

出　　处：《中国现代中药》2015年第6期572-576,共5页Modern Chinese Medicine

基　　金：国家科技支撑计划(2012BAD14B10);科技部国际合作项目(2013DFA30950);国家星火计划项目(2010GA860003);甘肃省民生计划项目(1209FCMA003);甘肃省青年科技基金计划(1208RJYA058);甘肃省科学院开发与应用基金项目(2012JK-03);甘肃省科学院青年基金项目(QN-2014-03)

摘　　要：目的:探索甘肃兴隆山桃儿七内生真菌的多样性和丰度,为鬼臼毒素的获取方式提供新途径,保护濒危药用植物桃儿七资源。方法:采用组织块培养法从一株桃儿七地下茎中分离纯化内生真菌,利用分子鉴定和常规的形态学方法对重复出现的4株内生真菌进行分类鉴定。结果:本研究采用组织块内生菌分离方法分离纯化了20株内生真菌,鉴定的4株菌分别是赭曲霉Aspergillus ochraceus,Eucasphaeria capensis,布氏白僵菌Beauvetia brongniaritii和镰孢菌Fusarium sp.。结论:内生真菌的多样性和丰度与桃儿七的生境有关,甘肃兴隆山桃儿七内生真菌优势属为Aspergillus,Eucasphaeria,Beauvetia和Fusarium。Objective： This study investigated the diversity and abundance of endophytic fungi of Sinopodophyllum hexandrum in Xinglong Mountain, Gansu Province, in order to provide a new way for obtaining podophyllotoxin from endophytic fungi, and protect endangered medicinal plants. Methods： The S. hexandrum endophytic fungi were isolated by tissue culture method, and identified with molecular and morphological data. Results： Twenty endophytic fungi were obtained from S. hexandrum underground stems, and four recurring endophytic fungi were identified as Aspergillus ochraceus, Eucasphaeria capensis, Beauvetia brongniaritii and Fusarium sp. Conclusion： Endophytic fungi diversity and abundance were related with host plants and environments, and the dominant endophytic genera in S. hexandrum in Xinglong Mountain are Aspergillus. Eucasphaeria. Beauvetia and Fusarium.

关 键 词：桃儿七 组织块培养法 内生真菌 分子鉴定 形态学鉴定 

分 类 号：R282.71[医药卫生—中药学]