一测双评法测定咳特灵胶囊中牡荆苷与异牡荆苷的含量  

Simultaneous Determination of Vitexin and Isovitexin of Keteling Capsules by Double-Components Assay with Single Marker

作　　者：员荣 孙丽丽 杨立伟 

机构地区：[1]广东药学院,广东广州510006 [2]广东省食品药品检验所,广东广州510180

出　　处：《中国现代中药》2015年第6期596-600,共5页Modern Chinese Medicine

基　　金：国家自然科学基金资助项目(81102146)

摘　　要：目的:建立咳特灵胶囊一测双评法,并进行方法学考察。方法:以牡荆苷为研究对象,建立牡荆苷与异牡荆苷的相对校正因子(RCF),并利用校正因子对异牡荆苷进行含量测定,实现一测双评;采用外标法测定牡荆苷与异牡荆苷的含量,比较计算值与实测值间的差异。结果:在一定线性范围内,牡荆苷与异牡荆苷的RCF为0.9,不同厂家的咳特灵胶囊中两个成分的计算值与实测值无明显差异。结论:本研究建立的一测双评法准确、可行,可为中药制剂多指标质量评价提供新思路。Objective： To establish a quantitative assay of double-components by single-marker for determination of two components in Keteling capsules and and conduct the method validation test. Methods： Vitexin was selected as an internal reference substance, RCF of isovitexin was calculated. The contents of two components were determined by both external standard method and Double-Components Assay by Single Marker. The validity of the QAMS method was evaluated by comparison of their quantitative results of both methods. Results： RCF of isovitexin with reference to vitexin were 0. 90 and the quantitative results of both external standard method and QAMS had no significant difference. Conclusion： The established method is accurate and feasible, and can provide a new mind for the quality assess of preparation of Chinese traditional medicine.

关 键 词：一测双评 校正因子 高效液相色谱 牡荆苷 异牡荆苷 

分 类 号：R974.2[医药卫生—药品]