糖肝康对糖尿病慢性肝损伤模型大鼠肝脏结构和细胞色素P450的影响  

The effect of Tanggankang on hepatic structure and cytochromes P450 in diabetic liver injury model rats

作　　者：钱秋海[1] 高彦彬[2] 彭伟[1] 钱卫斌[3] 蔡欣蕊[4] 郭春芳 武文广 姜群群[7] 

机构地区：[1]山东中医药大学附属医院内分泌科,山东250011 [2]首都医科大学中医药学院 [3]日本鸟取大学医学部 [4]山东中医药大学 [5]禹城市人民医院 [6]肥城市中医院 [7]中国人民解放军第107医院

出　　处：《现代中医临床》2015年第3期15-19,F0003,共6页Modern Chinese Clinical Medicine

基　　金：山东省自然科学基金面上项目(No.ZR2012HM093);山东省中医药科学技术研究项目(No.2011-038)

摘　　要：目的观察糖肝康对糖尿病慢性肝损伤大鼠肝脏组织光镜病理结构、电镜超微结构和肝功能、细胞色素P450(CYP450)的影响。方法将50只大鼠分为正常组、模型组、糖肝康小剂量组、糖肝康大剂量组、降糖甲组5组,每组10只。用链脲佐菌素和四氯化碳腹腔注射建立糖尿病慢性肝损伤大鼠模型。干预12周后,应用光镜和电镜观察各组大鼠肝脏病理结构和超微结构改变,比较各组大鼠肝功能,比较各组大鼠CYP450 1A1,CYP450 2E1,CYP450 3A1指标。结果光镜下,模型组大鼠肝细胞大量脂肪变性、增生有异型性,可见核分裂象,肝小叶间有少量纤维组织增生;糖肝康大剂量组肝脏结构基本正常。电镜下,模型组大鼠肝细胞体积小,核变形、皱缩,有切迹,核仁明显,常染色质团块状,异染色质边聚,线粒体肿胀,亦可见线粒体空泡化,线粒体嵴模糊;内质网肿胀;可见大量脂滴。糖肝康大剂量组肝细胞结构基本正常。糖肝康小剂量组与模型组相比,ALT明显降低(P<0.05);糖肝康大剂量组与模型组相比,ALT明显降低(P<0.01);降糖甲组与模型组相比,ALT明显降低(P<0.01);糖肝康大剂量组与降糖甲组相比,ALT明显降低(P<0.05)。糖肝康小剂量组与模型组相比,AST明显降低(P<0.01);糖肝康大剂量组与模型组相比,AST明显降低(P<0.01);降糖甲组与模型组相比,AST明显降低(P<0.05);糖肝康大剂量组与降糖甲组相比,AST明显降低(P<0.01)。糖肝康小剂量组、糖肝康大剂量组分别与模型组相比,CYP4501A1,CYP450 2E1,CYP450 3A1的表达均降低(P<0.05);降糖甲组与模型组相比,CYP450 1A1,CYP450 2E1,CYP450 3A1的表达均降低(P<0.05);糖肝康大剂量组与降糖甲组相比,CYP4501A1,CYP450 2E1,CYP450 3A1的表达均明显降低(P<0.05)。结论糖肝康对肝脏的病理改变起到一定改善作用,还可以通过调节肝功能,CYP450 1A1,CYP450 2E1,CYP450 3A1的表达,起到对肝脏的保护作用。Objective To observe the effect of Tanggankang on pathogenic structure of liver with light microscope, ultra structure with electron microscopy, and hepatic function, CYP50 in diabetic liver inju-ry model rats.Methods 50 rats were randomly divided into control group, model group, low-dose of Tanggankang group,high-dose of Tanggankang group and first group of hypoglycemic, with 10 rats in each group.The diabetic liver injury model rats were established with intraperitoneal injection of streptozotocin （ STZ） and carbon tetrachloride.After treated for 12 weeks, light microscopy and electron microscopy were used to detect pathogenic structure and ultra structure in rats.The hepatic function CYP450 1A1, CYP450 2E1,CYP450 3A1 among groups were compared.Results Under light microscopy, lots of fatty degerenerations of hepatocyte happened in model group, and the hyperplasia showed atypia, as well as mitotic figure, between the hepatic lobule, a small amount of fiber texture showed proliferation.In high-dose of Tanggankang group, hepatic structure showed essentially normal.Under electron microscopy, small volume hepatocyte, nuclear showed distortion and shrinking, incisures could be seen in nuclear, nucleolus was obvious seen, euchromatin was crumby and heterochromatin was dispersive, mitochondria was swollen, vacuolation mitochondria could also be seen, mitochondrial cristae was obscure, swollen en-doplasmic reticulum and lipid droplet were seen.In high-dose of Tanggankang group, hepatocyte showed essentially normal.Compared to model group, alanine transaminase （ ALT） of low-dose of Tanggankang group decreased significantly （ P〈0.05）;compared to model group, ALT of high-dose of Tanggankang group decreased significantly （ P〈0.01）;compared to model group, ALT of first group of hypoglycemic decreased significantly （P〈0.01）, compared to first group of hypoglycemic, ALT of high-dose of Tang-gankang group decreased significantly （ P 〈0.05）.Compared to model group, aspartate aminotrans-ferase （

关 键 词：糖肝康 慢性肝损伤 光镜 电镜 细胞色素P450 大鼠 

分 类 号：R285.5[医药卫生—中药学]