直链淀粉手性固定相拆分西那卡塞对映体  

Enantiomeric separation of cinacalcet on amylase chiral stationary phase by high performance liquid chromatography

作　　者：卢定强[1,2] 孙生柏 凌岫泉[1] 王琦[1] 夏芙洁 

机构地区：[1]南京工业大学药学院,江苏南京210009 [2]江苏省药物研究所,江苏南京210009

出　　处：《化学研究与应用》2015年第6期882-885,共4页Chemical Research and Application

摘　　要：建立了一种快速、准确的高效液相色谱分析方法。使用Chiralpak AD-H手性色谱柱,检测波长224nm,考察了流动相中极性调节剂的体积分数、流动相中三乙胺的体积分数、柱温及流速对西那卡塞对映体拆分的影响。确立了最佳拆分条件:流动相为正己烷-异丙醇-三乙胺(90∶10∶0.1,V/V/V);流速为1.0 m L·min-1;柱温为30℃。在此优化实验条件下,得到西那卡塞对映体的出峰顺序为:先S体,后R体。所建立的方法简单准确,重复性好,可用于西那卡塞的质量研究和控制。A quantitative analysis method for the determination of cinacalcet enantiomers by HPLC had been developed.The enantiomers of cinacalcet were separated on a Chiralpak AD-H column and the UV detection wavelength was 224 nm.The influences of concentration of organic solvent,proportion of triethylamine,column temperature and flow rate on the enantiomeric separation were investigated.The optimal chromatographic conditions were as follows： the mobile phase was hexane-isopropanol-triethylamine（90 ∶10∶0.1,V/V/V）;the flow rate was 1.0 m L·min-1;the column temperature was 30℃.Under the optimal conditions,the elution order of two enantiomers was S-cinacalcet before R-cinacalcet.It was simple,accurate,and reproducible HPLC method,and could be used for the analysis and quality control of cinacalcet.

关 键 词：西那卡塞 对映体拆分 Chiralpak AD-H 高效液相色谱 

分 类 号：O657.72[理学—分析化学]