哮喘小鼠模型中IL-25表达及其对肥大细胞IL-6分泌的影响  

Expression of IL-25 in asthmatic mice and its influence on release of IL-6 in mast cells

作　　者：徐红[1] 陈美元[1,2] 周瑶[1] 刘峰[1] 赵德育[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,江苏南京210008 [2]吴江区第一人民医院儿科,江苏苏州215200

出　　处：《南京医科大学学报（自然科学版）》2015年第6期804-807,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金青年项目(81200012);南京市医学科技发展项目(201108012)

摘　　要：目的 ：探讨哮喘小鼠模型中白细胞介素（interleukin,IL）-25的表达,研究其对肥大细胞IL-6分泌的影响。方法 ：6~8周龄BALB/c小鼠随机分为对照组和哮喘组,分别用等量磷酸盐缓冲液（phosphate buffer solution,PBS）或卵清蛋白（ovalbumin,OVA）致敏,检测肺泡灌洗液（bronchoalveolar lavage fluid,BALF）中细胞总数及嗜酸性粒细胞（eosnophils,EOS）计数验证模型的成功建立。两组小鼠肺组织中IL-25 m RNA、BALF及血清中IL-25的表达分别应用逆转录-聚合酶链反应法、酶联免疫吸附实验（enzyme-linked immunosorbent assay,ELISA）检测。小鼠肥大细胞P815分为对照组和IL-25组,分别予等量培养基和IL-25干预,收集细胞培养上清,用ELISA检测IL-6的水平。结果：哮喘组BALF中细胞总数及EOS计数较对照组显著升高（P〈0.01）,小鼠出现呼吸频率加快、烦躁不安等表现,模型建立成功。哮喘组肺组织中IL-25 m RNA、BALF及血清中IL-25表达较正常组显著升高（P〈0.01）。细胞水平的研究发现IL-25组P815上清中IL-6表达较对照组显著升高（P〈0.01）。结论：IL-25在OVA诱导哮喘小鼠模型中表达升高。IL-25刺激肥大细胞分泌IL-6,可能在哮喘的发病中起促进作用。Objective：To observe the expression of interleukin （IL）-25 in asthmatic mice and to investigate the potential influence of IL-25 on release of IL-6 in mast cells. Methods： BALB/c mice aged 6-8 weeks were randomly divided into the control group and the asthma group, and treated with equal amount of phosphate buffer solution（PBS） or ovalbumin（OVA）, respectively. Mouse asthma model was validated by detecting the total number of cells and eosinophil cells （EOS） in bronchoalveolar lavage fluid（BALF）. The expressions of IL-25 mRNA in lung tissues and IL-25 in BALF and serum were measured by reverse transcription-polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA） in the two groups. Mouse P815 cells were divided into the control group and the IL-25 group, and treated with equal amount of culture medium or IL-25, respectively. The levels of released IL-6 in supernatants were detect by ELISA. Results： In BALF, the total number of cells and EOS in the asthma group were significantly increased than those in the control group （P 〈 0.01）. The mice were irritable and their breathing rate were accelerated, and the model was validated. The expressions of IL-25 mRNA in lung tissues and IL-25 in BALF and serum were significantly higher in the asthma group than those in the control group（P 〈 0.01）. In P815 cell supernatants, the secretion of IL-6 in the IL-25 group were significantly higher than those in the control group（P 〈 0.01）. Conclusion： The expression of IL-25 was increased in OVA-induced mouse asthma model. IL-25 may promote the process of asthma by stimulating IL-6 release from mast cells.

关 键 词：白细胞介素-25 哮喘 小鼠 肥大细胞 白细胞介素-6 

分 类 号：R562.25[医药卫生—呼吸系统]