基于线粒体COⅡ基因序列的中华蜜蜂地理种群的遗传多样性研究  

Genetic diversity of geographic populations of Apis cerana cerana estimated by mitochondrial COⅡ gene sequences

作　　者：刘振国[1] 吉挺[1] 沈芳[1] 梁勤[2] 罗岳雄[3] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]福建农林大学蜂学院,福州350002 [3]广东昆虫研究所,广州510260

出　　处：《环境昆虫学报》2015年第3期567-575,共9页Journal of Environmental Entomology

基　　金：国家自然科学基金(31372382);江苏省科技支撑项目(SBE201230535)

摘　　要：通过分析我国20个不同地理种群中蜂线粒体COⅡ序列的变异,对中华蜜蜂群体遗传分化程度遗传多样性进行全面研究。结果表明：COⅡ基因部分序列中共发现16个变异位点和18个单倍型,核苷酸差异数的平均值为0.939,核苷酸分歧度（Dxy）在0.1%-0.965%之间变化,核苷酸遗传距离为-0.007%-1.489%。总种群的Fst为0.4978,差异均极显著（P〈0.001）。研究结果显示种群总体单倍型多样性较为丰富,种群间核苷酸分歧度差异很大。20个东方蜜蜂不同地理种群间存在显著的遗传分化。To investigate the level of genetic diversity and genetic differentiation among different populations and within populations of Apis cerana cerana in China,partial sequences of mt DNA CO Ⅱgene in 20 geographic populations from China were sequenced and analyzed. The result showed 16 variable sites and 18 haplotypes were observed in the sequence of mt DNA COⅡ. The average number of nucleotide divergence（ K） of CO Ⅱ were 0. 939. Inter- population Nucleotide Divergence（ Dxy） of COⅡ was ranged from 0. 1% to 0. 965%. Inter- population Net Nucleotide Divergence（ Da） in COⅡ was ranged from- 0. 007% to 0. 781%. The overall Fst value was 0. 4978,which indicated the genetic variation was extremely significant within populations（ P 〈0. 001）. These results indicated that the haplotype diversity was relative abundance,the Dxy was very different. There was significant divergence among 20 A. cerana populations.

关 键 词：中华蜜蜂 线粒体COⅡ 地理种群 遗传多样性 遗传分化 

分 类 号：Q966[生物学—昆虫学] S891.4[农业科学—特种经济动物饲养]