DNA条形码快速鉴定广州木槿蚜虫  

Rapididentification of aphids on Hibiscus syriacus Linn. in Guangzhou based on DNA barcoding

作　　者：汪珍春[1] 邓其芬 陈诗颖[1] 郑毅胜[1] 姜立云[2] 

机构地区：[1]广州大学生命科学学院,广州510006 [2]中国科学院动物进化与系统学重点实验室,北京100101

出　　处：《环境昆虫学报》2015年第3期583-590,共8页Journal of Environmental Entomology

基　　金：科技部基础工作专项(2011FY120200);国家基金委动物学特殊学科点(J1210002);国家级大学生创新训练项目(201311078013)

摘　　要：为快速、准确鉴定木槿蚜虫,本文在广州市30个采样点采集了木槿蚜虫样本64份,利用DNA条形码技术获得了64份线粒体细胞色素C氧化酶亚基I（COI）基因的658 bp序列,同时利用形态鉴定和DAN条形码鉴定确定其中共包括5种蚜虫,棉蚜Aphis gossypii Glover、绣线菊蚜Aphis spiraecola Patch、苹果蚜Aphis pomi de Geer、月季长管蚜Sitobion rosivorum（Zhang）和桃蚜Myzus persicae（Sulzer）。所有样品的种内平均遗传距离为0.30%（0-2.30±0.36%）,种间平均遗传距离为9.00%（3.30%-10.90±1.22%）。邻接法（neighbor-joining,NJ）构建的系统发育树显示采自30个采样点木槿上的5种蚜虫分别聚为一支,支持率达99%。所获DNA条形码对指导木槿的蚜虫防治有一定的指导意义。In order to identify the aphids rapidly and accurately in flower pest control,64 aphid species on Hibiscus syriacus Linn samples were gathered in 30 places from Guangzhou City. The 658 bp COI（ mitochondrial cytochrome c oxidase subunit I） sequences of 64 samples were amplified using universal barcoding primers,which belong to 5 species,Aphis gossypii Glover,Aphis spiraecola Patch,Aphis pomi de Geer,Sitobion rosivorum（ Zhang） and Myzus persicae（ Sulzer）. The results showed that the average intraspecies sequence divergences of COI is 0. 30%（ 0 - 2. 30 ± 0. 36%）,while the average interspecies sequence divergences is 9. 00%（ 3. 30% - 10. 90 ± 1. 22%）. Neighbor- joining phylogenetic tree was built on the COI gene sequences,and the results indicated that the same species was clustered in the same branch, approval rate is 99%. It suggested that DNA barcoding（ COI gene sequences） is applicable in the aphid identification from flowers.

关 键 词：蚜虫 DNA条形码 COI 快速鉴定 木槿 

分 类 号：Q969[生物学—昆虫学] S433.39[农业科学—农业昆虫与害虫防治]