有氧运动训练对D-半乳糖诱导的亚急性衰老大鼠血管老化的影响  

The Effects of Aerobic Exercise On The Subacute Aging Rats Induced By D-Galactose Vascular Aging

作　　者：任晓丽[1] 丁薇[2] 刘洁[2] 陈威[2] 楼永良[2] 

机构地区：[1]温州医科大学实验动物中心,浙江温州325035 [2]温州医科大学检验医学院,生命科学学院,浙江温州325035

出　　处：《广东微量元素科学》2015年第4期65-69,共5页Trace Elements Science

基　　金：浙江省教育厅基金资助项目(编号:Y201222989);浙江省科技计划项目(编号:2013C37007)

摘　　要：目的探讨游泳运动对大鼠血管抗衰老的影响。方法30只健康雄性SD大鼠（180～220g）随机分为3组：溶剂对照组（n=10），D-半乳糖组（n=10）和有氧运动＋D-半乳糖组（运动组，n=10）。D-半乳糖组和运动组大鼠每日颈部皮下注射D-半乳糖（125mg／kg），溶剂对照组大鼠每日颈部皮下注射同体积生理盐水，且运动组大鼠每日进行60rain的无负重游泳运动训练，持续8周。以血浆总一氧化氮合成酶（T—NOS）活力，一氧化氮（NO），内皮素（ET）和NO／ET平衡建立血管衰老指标评价体系，并测量主动脉组织超氧化物歧化酶、谷胱甘肽过氧化物酶（GSH—Px）、超氧化物歧化酶（SOD）和丙二醛（MDA）含量。结果与溶剂对照组相比，D-半乳糖组大鼠主动脉MDA和血浆ET含量显著升高，而GSH—Px、SOD活力、血浆NO含量和NO／ET比值显著下降。与D-半乳糖组相比，运动组大鼠主动脉MDA含量和T—NOS活力显著下降，GSH—Px、SOD、T—NOS活力、NO含量和NO／ET比值显著升高（P〈0．05），且与对照组相比上述各项指标无显著差异。结论D-半乳糖皮下注射可诱导大鼠主动脉脂质过氧化反应，血浆NO／ET失衡；适宜的有氧运动能显著增加大鼠血管的抗氧化能力，减少脂质过氧化反应，改善NO／ET失衡，预防血管衰老。Objective Discussion swimming movement effects on rat vascular aging. Methods 30 healthy male SD rats were randomly divided into 3 groups （180 -220 g） ： solvent control group （n = 10）, D -galactose group （n = 10） and aerobic exercise ＋ D - galactose group （ exercise group, n = 10）. D -galactose and exercise group rats daily neck subcutaneous injection of D - galactose solvent control group rats daily subcutaneously injected with saline solution volume, an （ 125 mg/kg）, d exercise group rats for 60 minutes daily without weight loading swimming exercise training, for 8 weeks. With total plasma nitric oxide synthase （NOS） T vitality, nitric oxide （NO）, endothelin （ET） and NO/ET balance vascular aging index evaluation system, and to measure the aorta tissue superoxide dismutase （ SOD）, glutathione peroxidase （ GSH - Px） and superoxide dismutase （SOD） and malondialdehyde （MDA） content. Results： Compared with solvent control group, D -galactose group rat aorta MDA and a significant rise in plasma ET levels, and GSH -px, SOD activity, the plasma NO levels and NO/ET ratio dropped significantly. Compared with D - galactose group, exercise group rat aorta MDA content and activity of the T - NOS were decreased significantly, GSH ,- px, SOD, T - NOS activity and NO content and NO/ET ratio significantly higher （P 〈 0. 05 ）, and there was NO significant difference compared with control group the above - mentioned indicators. Conclusion ： D - galactose subcutaneously to induce rat aorta lipid peroxidation, and serum NO/ET imbalance; Suitable aerobic exercise can significantly increase the oxidation resistance rats blood vessels, reduce lipid peroxidation, improvement of NO/ET imbalance, the prevention of vascular aging.

关 键 词：有氧运动 血管老化 心脑血管 

分 类 号：R54[医药卫生—心血管疾病]