大葱根系浸提液对樱桃萝卜幼苗的化感效应  

Allelopathic Effect of Allium fistulosum Root Extract on Raphanus sativus Seedling

作　　者：韩海霞[1] 姚岭柏[1] 黄玉山[1] 

机构地区：[1]集宁师范学院生物系,内蒙古乌兰察布012000

出　　处：《河南农业科学》2015年第6期105-108,共4页Journal of Henan Agricultural Sciences

基　　金：集宁师范学院科研课题(jsky2013035)

摘　　要：为了探明大葱茬地块对樱桃萝卜生长及生理机制的影响，采用不同质量浓度大葱根系水浸提液处理樱桃萝卜种子，进行发芽试验后测定樱桃萝卜幼苗的生长、抗氧化酶活性及丙二醛（ MDA）含量。结果表明：不同质量浓度大葱根系浸提液对樱桃萝卜幼苗化感效应有不同影响。大葱根系浸提液20~30 g/L对樱桃萝卜种子发芽、10~30 g/L对樱桃萝卜幼苗胚根长均表现为极显著的化感抑制作用，而〈10 g/L影响不明显，但对苗高始终表现为化感促进作用。与蒸馏水处理（对照）相比，随着大葱根系浸提液质量浓度的升高，过氧化物酶（ POD）活性先升高后下降，过氧化氢酶（ CAT）活性在浸提液10~30 g/L时极显著降低，而超氧化物歧化酶（ SOD）活性无显著变化；对POD、SOD、CAT活性的总化感作用抑制率统计表明，浸提液质量浓度为5~20 g/L时，酶总活性受化感作用促进，而30 g/L时则被抑制；浸提液5~30 g/L处理的MDA含量高于对照，其中20~30 g/L处理与对照差异显著。表明大葱根系浸提液对樱桃萝卜种子发芽及幼苗生长具有化感抑制作用。樱桃萝卜幼苗通过增强POD活性清除体内氧自由基，但是随处理浓度升高，抗氧化酶活性均受抑制，膜质过氧化程度加剧。In order to understand the effects of stubble of Allium fistulosum on growth and physiological mechanisms of Raphanus sativus,the growth,antioxidant enzyme activity and malonaldehyde（ MDA） con-tent of R. sativus seedling were measured by germination test in different concentrations of root extract of A. fistulosum. The results showed that there were different effects on the allelopathic effects of different concentrations root extract of A. fistulosum on R. sativus seedlings. The germination of R. sativus were in-hibited（P〈0. 01） by root extract of A. fistulosum when the concentration of extract were between 20 g/L and 30 g/L. The root length of R. sativus were inhibited（P〈0. 01） when the concentration of root extract were between 10 g/L and 30 g/L. And there were no significant difference when the concentration of root extract was less than 10 g/L compare with control. However,the seedling height was promoted by the root extract of A. fistulosum. With the increase of root extract concentration,the peroxide enzyme（ POD） activi-ty increased first and then decreased;and catalase（ CAT） activity decreased significantly when the con-centration of root extract were between 10 g/L and 30 g/L;there were no significant difference for the ac-tivities of superoxide dismutase（ SOD） between the root extract treatments and the control. And total ac-tivities of the three antioxidant enzymes were increased by allelopathy when the concentrations of root ex-tract were between 5 g/L and 20 g/L,and inhibited when the concentrations of root extract was 30 g/L. The contents of MDA were higher than the control when the the concentrations of root extract were be-tween 5 g/L and 30 g/L,and reaching significant levels from 20 g/L to 30 g/L. The above results showed that there was an allelopathic inhibition of A. fistulosum root extract on seed germination and seedling growth of R. sativus. Furthermore,oxygen radical in R. sativus were eliminated by increasing POD activi-ties. However,with the increase of root e

关 键 词：大葱根系浸提液 化感效应 樱桃萝卜 化感作用抑制率 抗氧化酶 丙二醛 

分 类 号：S633.1[农业科学—蔬菜学]