纳米尺度金棒诱导肿瘤细胞凋亡的机制研究  

Mechanisms of tumor cell's apoptosis triggered by gold nanorods

作　　者：张付雷 蒋成[2] 柯长洪[3] 余泰[4] 李素芬[5] 郭尚敬[1] 李威[1,2] 

机构地区：[1]聊城大学药学院,山东聊城252000 [2]第二军医大学国际肿瘤研究所,上海200433 [3]暨南大学,广东广州510632 [4]第二军医大学数字化课程中心,上海200433 [5]聊城大学生命科学院,山东聊城252000

出　　处：《中国生化药物杂志》2015年第4期42-44,47,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金委面上项目(31470964);科技部973分题(2012CB934002)

摘　　要：目的探究金纳米棒引发癌细胞凋亡的机制。方法利用金种子生长法合成金纳米棒,并对其进行表征;利用CCK-8评价金纳米棒对细胞的增殖抑制-毒性,并通过流式凋亡检测;利用JC-1对线粒体膜电位检测及western blot检测Caspase9和Caspase3的活化程度。结果通过研究发现金纳米棒对正常细胞毒性很小,而对肿瘤细胞毒性很大;并且随着金纳米棒作用时间增加癌细胞的凋亡比例也在增加;另外正常细胞线粒体膜电位没变化而癌细胞线粒体膜电位大幅度降低。结论金纳米棒引发的癌细胞凋亡是通过线粒体凋亡途径。Objective To explore the mechanism that gold nanorods trigger apoptosis in cancer cells.Methods Gold nanorods was synthesized by gold seed growing method, and its characterization was detected; gold nanorods on cell proliferation-toxicity were evaluated by CCK-8 Kit and apoptosis were detected by flow; mitochondrial membrane potential were tested by JC-1 and activation of Caspase 9 and Caspase 3 were detected by western blot. Results The results found that gold nanorods had nontoxic to normal cells, but highly toxic to tumor cells; and with the increasing of gold nanorods’ working time, the percentage of apoptotic cancer cells was increasing; in addition to, normal cells’ mitochondrial membrane potential did not change, but cancer cells had a significant reduction in mitochondrial membrane potential.Conclusion This study proves that gold nanorods induce apoptosis through the mitochondrial apoptosis pathway.

关 键 词：金纳米棒 线粒体 凋亡 

分 类 号：R979.19[医药卫生—药品]