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作 者:刘萍[1] 王雪瑞[1] 丁义峰[1] 王添乐[1] 张妍[1] 卢芳[1] 吕保鹤 范琪琪[1]
机构地区:[1]河南师范大学生命科学学院,河南新乡453007
出 处:《河南师范大学学报(自然科学版)》2015年第3期116-120,共5页Journal of Henan Normal University(Natural Science Edition)
基 金:河南省重点科技攻关计划项目(122102310356;142102110143)
摘 要:用不同质量浓度(0、3、6、9、12、15mg·L-1)的离子液体1-己基-3-甲基咪唑溴化盐([C6mim]Br)水溶液对番茄金顶一号品种进行浸种和苗期水培,研究了种子萌发、幼苗形态以及幼苗叶片生理生化的变化.结果表明,当离子液体质量浓度9mg·L-1时,种子发芽率比对照降低差异显著;当离子液体质量浓度6mg·L-1时,根长和苗长比对照降低差异极显著.本研究所设的所有离子液体质量浓度均使番茄幼苗叶片中叶绿素含量比对照降低差异显著,尤其是当9mg·L-1时则差异极显著.幼苗叶片超氧化物歧化酶(SOD)在离子液体质量浓度为6~12mg·L-1和15mg·L-1时分别比对照降低达显著与极显著水平,当离子液体质量浓度12mg·L-1时,POD活力比对照降低差异极显著.处理组幼苗叶片中超氧阴离子(O2·-)产生速率和丙二醛(MDA)含量分别在离子液体质量浓度6mg·L-1和9mg·L-1时比对照增加,达极显著差异水平.在本试验条件下,离子液体1-己基-3-甲基咪唑溴化盐对番茄种子萌发和幼苗生长均具有明显的抑制效应.Effects of different concentrations of 1-hexyl-3-methylimidazolium bromide ionic liquid([C6 mim]Br) (0,3,6, 9,12,15 mg L 1) on the germination rate of tomato seeds and growth of seedlings were evaluated by hydroponics. In this study, the changes of seed germination, seedling morphology and physiological and biochemical changes in seedling leaves were analyzed. The results showed that seed germination rate was significantly reduced when the concentration of ionic liquid 9 mg L- compared with the control group; And when the concentration of ionic liquid6 mg L 1, root length and shoot length were significantly lower than those of the control. Compared with the control group, chlorophyll contents of tomato seedlings leaves were significantly decreased after treatment of all ionic liquid. And it is extremely significant difference when 9 mg L-. Superoxide dismutase'(SOD) in ionic liquid concentration is 6--12 mg I.- and 15 mg L- were lower than those of the control were significant and extremely significant level. The activity of POD were significantly lower than those of the control when the concentration of ionic liquid 〉12mg L 1. When the concentration of ionic liquids6 mg IJ and 9 mg g-1 , the superoxide anion (O2 - ) production rate and malonaldehyde (MDA) content in leaves of treatment group were significantly greater than control group and up to a very significant difference. Under this experimental condition, the effect of ionic liquid 1-3-imidazolium bromide salt on the germination and seedling growth of tomato seeds were inhibited significantly.
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