毛细管电色谱-电喷雾-飞行时间/质谱联用分离分析混合手性药物  

Separation and Analysis of Chiral Drugs Mixture by Capillary Electrochromatography-Electrospray-Time of Flight / Mass Spectrometry

作　　者：李英杰[1] 徐红梅[1] 高立娣[1] 吕仁江[1] 于春杰[1] 王珊珊[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《分析测试学报》2015年第6期691-695,共5页Journal of Instrumental Analysis

基　　金：黑龙江省自然科学基金资助项目(41465500-7-12042)

摘　　要：建立了一种毛细管电色谱-电喷雾-飞行时间/质谱(CEC-ESI-TOF/MS)联用分离分析盐酸异丙肾上腺素和盐酸氯丙那林混合手性药物的方法。利用实验室自制有机-无机杂化开管柱作为色谱分离柱,考察了缓冲溶液的浓度、p H值、运行电压、分离温度、鞘液的种类、鞘液添加剂、鞘液的流速等分离检测条件对分离度和电离强度的影响。结果表明,在优化的分离检测条件下,两种混合手性药物的4个组分在18.5min内实现基线分离。A capillary electrochromatography- electrospray- time of flight / mass spectrometric（ CEC-ESI- TOF / MS） method for the separation and analysis of chiral drugs mixture of isoprenaline hydrochloride and clorprenaline hydrochloride was established. A home made organic- inorganic hybrid open column was used as chromatographic separation column. The influences of separation and detection conditions including buffer concentration,p H value,operation voltage,separation temperature,sheath fluid type,sheath fluid additives and flow rate of the sheath fluid on resolution and ionization intensity were investigated. The results showed that the baseline separation of four components of two chiral drugs mixture were achieved within 18. 5 min under the optimized conditions.

关 键 词：毛细管电色谱-电喷雾-飞行时间/质谱 盐酸异丙肾上腺素 盐酸氯丙那林 混合手性药物 手性分离 

分 类 号：O657.1[理学—分析化学] Q522.4[理学—化学]