逆流色谱分离中选择性试剂的应用研究进展  

Progress on Application of Different Selective Reagents in Counter-current Chromatography

作　　者：鲁梦霞 熊晴[1] 王小平[1] 颜继忠[1] 童胜强[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《分析测试学报》2015年第6期744-748,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21105090);浙江省高等学校中青年学科带头人攀登项目(pd2013031)

摘　　要：逆流色谱中添加选择性试剂可有效提高分离度,常用选择性试剂主要有金属离子、多糖衍生物、阴离子表面活性剂、离子对、手性试剂、p H-区带逆流色谱中酸碱试剂等6类。该文综述了国内外选择性试剂在逆流色谱分离中的应用研究进展,介绍了其基本原理,阐述了不同选择性试剂的适用范围,并展望了选择性试剂发展的新趋势。Using selective reagents in the separation technique of complexation counter-current chro- matography can improve the resolution effectively. The commonly used selective reagents include met- al ion selective reagent, polysaccharideion selective reagent, anionic surface active agent, ion pair selective reagent, chiral reagent and acid-base reagents of pH - zone - refining. In this paper, the applications of different kinds of selective reagents in counter-current chromatography were summa- rized. The fundamental principle was introduced, and the way to improve the resolution was ana- lyzed. Finally, the scope of application and the development trend of each selective reagents were al- so discussed.

关 键 词：逆流色谱 选择性试剂 基本原理 综述 

分 类 号：O657.7[理学—分析化学] G353.11[理学—化学]