利用Pi9基因分子标记辅助选择培育抗稻瘟病水稻新品系  

Breeding New Rice Line with Blast Resistance by Molecular Marker-assisted Selection of the Pi9 Gene

作　　者：谭令辞 刘雄伦[1,2] 杨婷婷[1,2] 行璇 梁毅 陈海龙[1,2] 杨丰宇[1,2] 李永聪[1,2] 刘金灵[1,2] 戴良英[2,4] 陈立云[1] 王国梁[1,2] 

机构地区：[1]湖南农业大学农学院,长沙410128 [2]作物基因工程湖南省重点实验室,长沙410128 [3]中国种子集团有限公司,湖北武汉430206 [4]湖南农业大学植物保护学院,长沙410128

出　　处：《作物研究》2015年第4期348-351,356,共5页Crop Research

基　　金：国家自然科学基金(3l171526);国家转基因生物新品种培育重大专项(2014ZX08001-002);教育部高校创新团队发展计划(IRT1239)

摘　　要：利用广谱持久抗瘟基因Pi9的内含子序列设计特异功能标记Ins2-1,通过分子标记辅助选择和连续回交育种,改良早籼稻1701的稻瘟病抗性。结果表明:Ins2-1是一个显性分子标记,在Pi9基因供体亲本75-1-127和受体亲本1701之间多态性明显且稳定;Ins2-1标记基因型对稻瘟病抗性表型的选择效率为100%;通过连续回交和自交,获得了含Pi9基因且遗传背景接近受体亲本的BC6F2群体;通过对BC6F3株系标记基因型和室内接种表型鉴定,筛选出了一个抗病纯系"抗瘟1701"。To improve blast resistance of the early season indica rice line 1701, a dominant functional DNA marker, Ins2-1, was developed based on the intron sequence of the broad-spectrum blast resistance gene Pi9 and marker-assisted selection breeding practice was implemented in this study. The main results were as follows： Ins2-1 showed stable polymotphism be- tween 1701 and 75-1-127,the Pi9 donor, and the selection efficiency of the marker genotype for blast resistance phenotype was 100%. A BC6F2 population,harboring the Pi9 gene and consecutive backcrossing and bagged-pollination,and a blast possessing receptor＇ s genomic background,was developed by resistant rice pure line, named resistant 1701 ,was identified from the BC6 F3 families by genotypic and phenotypic analyses, these work paved a way for breeding elite blast resistant rice varieties or hybrids.

关 键 词：水稻 稻瘟病 Pi9基因 分子标记辅助选择育种 

分 类 号：S511.034[农业科学—作物学]