UHPLC法同时测定不同产地葛根中5种活性成分的含量  

Simultaneous Determination of Five Active Components in Puerariae Lobatae Radix from Different Habitats by UHPLC

作　　者：赵路[1] 关琴笑[1] 路丽[1] 郭先帅 刘国红[1] 王淑美[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《中药材》2015年第3期473-475,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81073024;81050019)

摘　　要：目的：建立同时测定不同产地葛根中3＇-羟基葛根素、葛根素、3＇-甲氧基葛根素、大豆苷和大豆苷元5个成分含量的UHPLC法。方法：采用Agela Venusil MP C18（100 mm×2.1 mm,3μm）色谱柱,以甲醇-0.1%甲酸梯度洗脱,检测波长250 nm。结果：3＇-羟基葛根素、葛根素、3＇-甲氧基葛根素、大豆苷和大豆苷元分别在12.41-248.24ng（r=0.9999）、58.82-1 176.47 ng（r=0.9997）、12.65-252.94 ng（r=0.9999）、12.14-242.82 ng（r=0.9998）、1.82-36.30 ng（r=0.9997）进样量范围内与峰面积呈良好的线性关系;平均加样回收率为99.03%-100.32%,RSD值为0.26%-1.37%。结论：本方法简便可靠、快速,可有效控制葛根的质量。Objective： To develop a UHPLC method for determination of 3＇-hydroxy puerarin,puerarin,3＇-methoxy puerarin,daidzin,and daidzein in Puerariae Lobatae Radix from different habitats. Methods： The analysis was carried out on an Agela Venusil MP C18（ 100 mm × 2. 1 mm,3 μm） column. The mobile phase was composed of 0. 1% formic acid and methanol with gradient elution. The detection wavelength was set at 250 nm. Results： The standard curves of five components showed a good linearity in 12. 41 - 248. 24 ng（ r= 0. 9999）,58. 82 - 1 176. 47 ng（ r = 0. 9997）,12. 65 - 252. 94 ng（ r = 0. 9999）,12. 14 - 242. 82 ng（ r = 0. 9998）,and 1. 82 - 36. 30ng（ r = 0. 9997）,respectively. The average recoveries were 99. 03% - 100. 32%,and RSD values were 0. 26% - 1. 37%. Conclusion：This method is simple,quick,reproducible and can be used to control the quality of Puerariae Lobatae Radix.

关 键 词：超高效液相色谱法 葛根 活性成分 

分 类 号：R284.1[医药卫生—中药学]