亳菊HPLC特征指纹图谱研究  

HPLC Characteristic Fingerprint of “Boju”Chrysanthemum morifolium

作　　者：俞年军[1,2] 于娇[1,2] 张伟[1,3] 曹勇[3] 郑太华 王韵琴[3] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230031 [2]中药资源保护与开发研究所,安徽合肥230031 [3]安徽济人药业有限公司,安徽亳州236800

出　　处：《中药材》2015年第3期497-500,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技部十二五支撑计划项目(2011BAI04B04);国家自然科学基金青年项目(81303229)

摘　　要：目的:建立亳菊的HPLC特征指纹图谱,为亳菊的质量控制提供依据。方法:采用Spursil C18色谱柱(250 mm×4.6 mm,5μm);乙腈(A)-0.1%磷酸(B)为流动相,流速1.0 m L/min,进行梯度洗脱;检测波长325 nm,柱温30℃。结果:建立了亳菊HPLC指纹图谱的共有模式,标定16个共有峰,利用对照品指认5个峰,10批亳菊样品的HPLC图谱相似度均大于0.900。结论:所建立的HPLC指纹图谱分析方法简单、重现性良好,可为亳菊的品种鉴别及质量评价提供依据。Objective： To provide a scientific basis for quality control of＂Boju＂Chrysanthemum morifolium by establishing a HPLC characteristic fingerprint. Methods： The HPLC analysis was performed on a Spursil C18 chromatographic column（ 250 mm × 4. 6 mm,5μm）,and the mobile phase was acetonitrile-0. 1% phosphoric acid in a gradient mode with the flow rate of 1. 0 m L/min. The detection wavelength was 325 nm and the temperature of column was 30 ℃. Results： The common pattern of HPLC characteristic chromatographic profile was established. There were 16 common peaks,five of which were identified in the pattern. The similarities of 10 batches of＂Boju＂Chrysanthemum morifolium were evaluated,and all of them were greater than 0. 900. Conclusion： This analysis method of HPLC characteristic chromatographic fingerprint is simple and reproducible,and it can provide a scientific basis for identification and quality evaluation of ＂Boju＂Chrysanthemum morifolium.

关 键 词：亳菊 HPLC指纹图谱 相似度 

分 类 号：R282.5[医药卫生—中药学]