丹皮酚对酒精性脂肪肝大鼠的治疗作用  

Therapeutic Effect of Paconol on Alcoholic Fatty Liver in Rats

作　　者：颜贵明[1] 戴敏[1] 宣自华[1] 

机构地区：[1]省部共建新安医学教育部重点实验室/安徽中医药大学,安徽合肥230012

出　　处：《中药材》2015年第3期550-555,共6页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划(2006BAI06A18-07)

摘　　要：目的:明确丹皮酚对酒精性脂肪肝(AFLD)的治疗作用。方法:采用单纯酒精灌胃造模方法复制AFLD大鼠模型,以丹皮酚(75、150、300 mg/kg)灌胃治疗4 w,水飞蓟宾作为阳性对照药。4-氨基安替比林法(4-AAP)检测血清和肝脏胆固醇(TC)、甘油三酯(TG)等脂质的含量;赖氏法测定血清丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)水平;直接法测定血清高密度脂蛋白(HDL)的含量;沉淀法测定血清低密度脂蛋白(LDL)含量;双抗体夹心ELISA法检测血清TNF-α含量;酶比色法检测血清和肝脏组织FFA含量;硫代巴比妥法测定血清MDA含量;SABC法测定肝脏CYP2E1蛋白表达;并从肉眼和在光镜下观察肝脏的病理学变化。结果:丹皮酚可降低模型动物血清TC、TG、HDL、LDL、ALT、AST、TNF-α、FFA、MDA及肝组织中TC、TG、FFA水平,抑制CYP2E1蛋白表达,并能改善AFLD大鼠肝脏形态及组织病理学异常。结论:丹皮酚对酒精性脂肪肝大鼠具有一定的治疗作用。Objective： To make clear the therapeutic effect of paconol on alcoholic fatty liver disease（ AFLD）. Methods： Model rats of AFLD were established with alcohol intragastric administration. Paconol was applied to treat the model rats for four weeks（ 75,150 and 300 mg / kg）,with silybin as control administration. The content of TC and TG in serum and liver were determined with 4-Aminoantipyrine（ 4-AAP） method,ALT and AST levels were determined with Reitman-Frankel method,serum HDL content with direct method,serum LDL content with precipitation method,serum TNF-α content with enzyme linked immunosorbent assay sandwich technique,FFA content in serum and liver with enzymic colorimetric method,MDA content with thiobarbituric acid reactive substance assay method,liver CYP2E1 expression with SABC method,and the pathological changes of liver were observed directly or with optical microscope. Results： Paconol lowered TC,TG,HDL,LDL,ALT,AST,TNF-α,FFA and MDA levels in serum,as well as TC、TG and FFA levels in liver,inhibited the expression of protein CYP2E1,and improved the pathological changes of model rats. Conclusion： There is a certain therapeutic effect of paconol on AFLD in rats.

关 键 词：丹皮酚 酒精性脂肪肝 

分 类 号：R285.5[医药卫生—中药学]