瓜蒌薤白滴丸及其组方药味的药效学研究  

Pharmacodynamics Study on Gualou Xiebai Dropping Pills and Its Medicinal Ingredients in Prescription

作　　者：鄢海燕[1] 邹纯才[1] 魏美玲[1] 高征征 杨洋[1] 

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《中药材》2015年第3期567-571,共5页Journal of Chinese Medicinal Materials

基　　金：安徽省科技攻关项目(12010402089);国家大学生创新创业训练计划项目(201310368042;201410368013);皖南医学院重点培育基金项目(WK2014Z04)

摘　　要：目的:对瓜蒌薤白滴丸及其组方药味的抗心肌缺血药效进行研究。方法:将SPF级SD大鼠随机分为11组,每组10只,雌雄各半,正常对照组与模型组灌胃生理盐水,药物组9组,分别灌胃给予瓜蒌、薤白及瓜蒌薤白白酒汤(均相当于生药22.5 g/kg),瓜蒌薤白滴丸3.75、11.25、22.5、33.75、45 g/kg及复方丹参滴丸0.085 g/kg,1次/d,干预7 d后,除正常对照组外,其余各组腹腔注射ISO制备大鼠急性心肌缺血模型,观察各组大鼠心电图ST段的变化;测定血浆SOD、NO、HDL-C、MDA、CAT、LDH、CK水平;对心肌组织进行HE染色,观察组织病理变化。结果:各给药组与模型组比较,瓜蒌薤白滴丸22.5、33.75、45 g/kg组分别有10、11、12个时间点ST段明显回落(P<0.05),回落时间点数多于其他各组;瓜蒌薤白滴丸22.5、33.75 g/kg能同时显著改善模型大鼠血浆SOD、MDA、CAT、NO、HDL-C、LDH及CK水平(P<0.05或P<0.01);瓜蒌薤白滴丸各剂量均能改善模型大鼠心肌组织病理学改变。结论:瓜蒌薤白滴丸能有效对抗ISO所致的大鼠急性心肌缺血,效果优于瓜蒌、薤白单用及瓜蒌薤白白酒汤。Objective： To study the pharmacodynamics of Gualou Xiebai Dropping Pills and its medicinal ingredients in prescription on anti-myocardial ischemia. Methods： SPF Rats were divided randomly into eleven groups with ten rats in each group and half male and half female,the rats were respectively given the physiological saline（ blank group and model group）,Gualou,Xiebai,Gualou Xiebai Baijiutang（ all equivalent to the crude herb of 22. 5 g / kg）,Gualou Xiebai Dropping Pills in the doses of 3. 75,11. 25,22. 5,33. 75 and 45 g / kg and Compound Danshen Drop Pills of 0. 085 g / kg by gavage one time a day for seven days. Except blank group,other rats were given by intraperitoneal injection of isoproterenol to establish myocardial ischemia models,changes of ST segments in ECG were observed in all groups,and the levels of SOD,NO,HDL-C,MDA,CAT,LDH and CK in blood plasma were detected,and the pathological changes of myocardial tissues were observed under light microscope by HE staining. Results： Compared with model group,ST segments in ECG dropped markedly at different time point which included 10,11 and 12（ P 0. 05） in Gualou Xiebai Drop Pills groups of 22. 5,33. 75 and 45 g / kg,time points were more than those of other groups. Gualou Xiebai Dropping Pills groups of 22. 5 and 33. 75 g / kg improved the levels of SOD,MDA,CAT,NO,HDL-C,LDH and CK in blood plasma in model rats significantly（ P 0. 01 or P 0. 05）.Gualou Xeibai Dropping Pills improved the pathological changes of myocardial tissues at all dosages. Conclusion： Gualou Xiebai Drop Pills can effectively restrain the acute myocardial ischemia induced by isoproterenol in rats,compared with Gualou,Xiebai or Gualou Xiebai Baijiutang,Gualou Xiebai Drop Pills obtains a favourable effect.

关 键 词：瓜蒌薤白滴丸 组方药味 抗心肌缺血 药效 

分 类 号：R285.5[医药卫生—中药学]