FHL1在低氧/炎症诱导形成肺动脉高压作用中的初步研究  

A preliminary study of the role of fhl1 in pulmonary hypertension induced by hypoxic/inflammation

作　　者：王海滨[1] 杨艳娟[2] 郑西卫[2] 杨桂兰[1] 訾瑞[1] 王婷[1] 

机构地区：[1]宁夏医科大学研究生院,宁夏银川750004 [2]宁夏医科大学总医院呼吸科,宁夏银川750004

出　　处：《宁夏医学杂志》2015年第6期508-510,F0002,共4页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ1202)

摘　　要：目的了解四个半LIM结构域蛋白1(FHL1)在低氧/炎症条件下在肺血管内的表达及其与肺血管重塑的关系。方法将40只Wistar雄性大鼠分为对照组、低氧组、野百合碱组、低氧+野百合碱组,每组10只,分别测定各组大鼠平均肺动脉压力(m PAP)、右心室肥厚指数(RVHI)及肺小动脉相应管壁中膜厚度占其管径的百分比(WT%)。免疫组化法检测各组大鼠肺内FHL1的表达,并对肺组织切片进行图像分析。结果低氧组、野百合碱组及低氧+野百合碱组大鼠的m PAP与对照组相比均显著升高(P<0.05),3组的RVHI与对照组相比均升高(P<0.05),3组的WT%与对照组相比均显著升高(P<0.05)。低氧组、野百合碱组及低氧+野百合碱组3组大鼠的肺动脉FHL1免疫阳性染色分别为0.16±0.03、0.15±0.03、0.21±0.03,与对照组的0.08±0.02相比明显增强(P<0.05),低氧+野百合碱组大鼠的肺动脉FHL1表达较低氧组及野百合碱组相比增强(P<0.05)。结论在缺氧/炎症诱导肺动脉高压形成的过程中FHL1表达增加,可能在肺血管重塑和肺动脉高压的发病过程中发挥一定的作用。Objective To investigate the expression of FHL1 and the role of it in the occurrence of pulmonary vascular remode- ling under the condition of low oxygen/inflammation. Methods 40 male Wistar rats were randomly divided into four group （ n= 10 ） ： control group,hypoxic model group, monoerotaline injection model group （MCT group ） and monocrotaline injection while low oxygen treatment model group. The mean pulmonary arterial pressure （mPAP） ,WT% and RVHI were measured. The expression of FHL1 in the lungs of rats were measured by immunohistochemical staining. Results The mPAP of hypoxic model group, MCT group and monocrotal- inel injection while low oxygen treatment model group were significantly increased compared with the control group （ P 〈 0.05 ）, those groups of RVHI were higher compared with the control group （ P 〈 0.05 ）. WT% of the three groups were significantly higher than that the control group （P 〈 0.05 ）. The positive staining of FHL1 of the three groups （0.16 ± 0.03,0.15 ± 0.03 and 0. 21±0. 03 ） was sig- nificantly increased compared with the control group （0.08 ± 0.02）. The expression of FHL1 in the monocrotalinel injection while low ox- ygen treatment model group was increased with hypoxic model group or MCT group （ P 〈 0.05 ）. Conclusion FHL1 may play an impor- tant role in the pathogenesis of pulmonary vascular remodeling and pulmonary hypertension.

关 键 词：肺动脉高压 血管重塑 低氧 炎症 结构域蛋白1 

分 类 号：R544.1[医药卫生—心血管疾病]