绿原酸调节肥胖大鼠胰岛素抵抗作用的研究  

Mechanism research of chlorogenie acids on insulin resistance in nutritional obese rats

作　　者：杨辉[1] 周远大[2] 何海霞[2] 

机构地区：[1]重庆医科大学附属第一医院药学部,400016 [2]重庆医科大学附属第一医院临床药理研究室,400016

出　　处：《重庆医学》2015年第18期2457-2460,共4页Chongqing medicine

基　　金：重庆市教委科研基金资助项目(KJ110319)

摘　　要：目的研究绿原酸(CHA)对高脂诱导的肥胖大鼠胰岛素抵抗(IR)的调节作用。方法采用饲喂高脂饲料的方法建立营养型肥胖大鼠IR模型,将造模成功的大鼠分为4组:模型组、吡格列酮组(4.5mg/kg)、CHA(大、小剂量组),以未造模的正常大鼠作为空白组,连续给药4周后,测定其糖耐量、血清胰岛素、血脂水平等指标。结果 CHA能显著减缓肥胖大鼠体质量的增速,降低血糖浓度时间曲线下面积(AUC),增加糖耐量,降低血清胰岛素、三酰甘油和总胆固醇水平,增加肝糖原、肌糖原水平,降低胰岛素敏感指数(HOMA-IR),升高胰岛素抵抗指数(HOMA-ISI)。结论 CHA具有调节肥胖大鼠IR的作用,其机制与抑制内脏脂肪聚积,降低体内肝糖原、肌糖原水平,促进外周组织吸收利用葡萄糖,降低血清游离脂肪酸(FFA)水平,防止脂质过氧化有关,其分子机制值得进一步研究。Objective Study the effect of chlorogenie acids（CHA）on insulin resistance in obese rats induced by high‐fat diet . Methods We induced the obese rat model by feeding high‐fat diet ,obese rat model were divided into 4 groups：model group ,piogli‐tazone group（4 .5 mg/kg） ,CHA large dose group and group ,and finally determinated the levels of glucose tolerance ,serum insulin , serum lipid profiles and others .Results CHA showed a higher anti‐obesity activity with lower rate of increase of obese rats′body weights ,reversingglucose intolerance induced by high‐fat diet ,ameliorating the hyperinsulinemia ,decreaseing the levels of TG and TC ,and increase liver glycogen and muscle glycogen level compared with other group which treated with high‐fat diet .And in‐creased HOMA‐ISI ,decreased HOMA‐IR .Conclusion CHA can ameliorate the symptoms of insulin resistance in obese rats ,which mechanism may be related with CHA can stimulate glucose uptake and utilization by peripheral tissues ,and decrease the the serum levels of FFA ,decrease oxygen stress ,prevent and cure the injury induced by lipid peroxidation .

关 键 词：绿原酸 大鼠 近交系 胰岛素抗药性 

分 类 号：R285.5[医药卫生—中药学]