培哚普利下调糖尿病大鼠肾小管间质清道夫受体A表达  

Perindopril down-regulates scavenger receptor A expression in renal tubulointerstitium of diabetic rats

作　　者：曾映娟[1] 孙莹[1] 文江华[1] 胡芳[1] 罗顺葵[1] 鲁红云[1] 

机构地区：[1]中山大学附属第五医院内分泌科,广东珠海519000

出　　处：《重庆医学》2015年第18期2461-2463,共3页Chongqing medicine

摘　　要：目的探讨培哚普利对链脲佐菌素(STZ)诱导的大鼠糖尿病模型肾小管间质中清道夫受体A(SR-A)表达的影响。方法将大鼠随机分为健康对照组、糖尿病组和培哚普利干预组。检测各组大鼠的血糖和尿清蛋白,行Masson染色观察肾脏形态学变化,应用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学检测SR-A mRNA及蛋白的表达。结果与健康对照组比较,糖尿病组大鼠血糖和24h尿清蛋白均显著上升;肾组织SR-A mRNA和蛋白表达均显著上调,培哚普利干预后,上述指标除血糖外均明显被抑制(P<0.05)。结论糖尿病大鼠肾小管间质SR-A表达增加,培哚普利对糖尿病大鼠肾脏有保护作用,其机制可能部分与下调肾小管间质SR-A表达有关。Objective To investigate the effect of an ACE inhibitor ,perindopril ,on the expression of SR‐A in renal tubuloint‐erstitium of diabetic rats .Methods Diabetes was induced in male Sprague‐Dawley rats by injection with streptozotocin .The rats were then randomly divided into 3 groups：normal control group;untreated diabetes mellitus group and diabetes mellitus group trea‐ted with perindopril .After a 24‐week treatment ,tubulointerstitial injury index was assessed with Masson′s trichrome sections .The expression of SR‐A mRNA was detected by RT‐PCR and the expression of SR‐A protein in renal tubulointerstitium was detected by immunohistochemistry .Results The tubulointerstitial injury index ,the expression of SR‐A mRNA were significantly higher in the diabetes group than those in normal control group .Perindopril treatment not only attenuated the tubulointerstitial injury ,but also reduced the overexpression of SR‐A mRNA in diabetic rats .The expression of SR‐A protein was most obvious in renal tubulointer‐stitium in diabetic rats ,which was obviously attenuated by perindopril treatment（P〈0 .05） .Conclusion The findings of the this study indicate that perindopril may have renoprotective effects on diabetic nephropathy via inhibiting the expression of SR‐A in re‐nal tubulointerstitium .

关 键 词：糖尿病肾病 培哚普利 清道夫受体  A类 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]