重组人促红细胞生成素对急性脑损伤大鼠的神经保护作用研究  

The protection study of recombinant human erythropoietin on acute cerebral injury rats

作　　者：于代华[1] 孙绪德[1] 柴伟[1] 高昌俊[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安710038

出　　处：《重庆医学》2015年第18期2474-2476,共3页Chongqing medicine

摘　　要：目的探讨重组人促红细胞生成素(rh-EPO)对大鼠急性脑损伤后脑细胞凋亡的保护作用及对GLT-1/GLAST表达的影响。方法 60只大鼠分为对照组(n=18)、急性脑损伤组(n=22)和rh-EPO处理组(n=20),急性脑损伤组用改良Feeney′s自由落体打击器制作急性脑损伤模型,rh-EPO处理组采用同样的方法制作脑外伤模型15min后腹腔注射rh-EPO,所有动物在实验完成48h后断头取脑。应用RT-PCR、Western blot检测GLT-1、GLAST mRNA及蛋白表达。结果急性脑损伤48h后,大鼠缺血区GLT-1和GLAST mRNA及蛋白表达较对照组均显著降低(均P<0.01),而rh-EPO处理组GLT-1和GLAST的mRNA及蛋白表达较急性脑损伤组均显著增加(均P<0.01)。结论 EPO可显著减轻大鼠急性脑损伤,其机制可能与GLT-1/GLAST表达上调有关。Objective To investigate the effect of recombinant human erythropoietin（rh‐EPO ） on acute cerebral injury and expression of GLT‐1 and GLAST in rat .Methods Sixty SD rats were randomly divided into three groups by weight ：control group （n=18） ,acute cerebral injury group（n=22） and rh‐EPO conditioning group（n=20） .Acute cerebral injury models were made by modified Feeney′s method .rh‐EPO was injected in abdominal cavity 15 min after acute cerebral injury in rh‐EPO conditioning group .Rats′brain were removed 48 h after experiments .Rat GLT‐1 and GLAST mRNA expression were determined by RT‐PCR , GLT‐1 and GLAST protein expression were determined by Western blot .Results GLT‐1/GLAST mRNA and protein expression decreased significantly after acute cerebral injury（all P〈0 .01） ,but increased significantly in rh‐EPO preconditioning group com‐pared with acute cerebral injury group（all P〈0 .01） .Conclusion rh‐EPO preconditioning may protect against acute cerebral injury by up regulating the expression of GLT‐1/GLAST .

关 键 词：红细胞生成素 脑损伤 谷氨酸转运体1 天冬氨酸氨基转移酶类 

分 类 号：R651.15[医药卫生—外科学]