不同抗性苜蓿株系叶片感染假盘菌后酶活性的变化  

Changes of Enzyme Activity in Leaves of Alfalfa Lines with Different Resistance Infected with Pseudopeziza medicaginis(Lib.)Sacc.

作　　者：黄海燕[1,2] 王瑜[1] 袁庆华[1] 李跃[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]甘肃农业大学,甘肃兰州730070

出　　处：《中国草地学报》2015年第3期55-59,共5页Chinese Journal of Grassland

基　　金："十二五"国家科技支撑计划项目(2011BAD17B01)

摘　　要：采用活体整株接菌法研究苜蓿高抗褐斑病株系DR1111和高感株系DS10在假盘菌诱导不同时间（0d、1d、2d、3d、4d、5d、10d、15d、20d）后叶片多胺氧化酶（PAO）、二胺氧化酶（DAO）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性变化.结果表明,接种假盘菌后高抗病株系和高感病株系的四种酶活性均有提高,接菌后20d内酶活性变化基本上呈先升高再降低的趋势,高抗株系的DAO活性显著高于高感株系（P＜0.05）,感病株系的SOD活性在第3～10d显著高于抗病株系（P＜0.05）;高抗株系的PAO和DAO活性峰值出现早于高感株系,而高抗株系的CAT活性峰值则晚于高感株系.High resistant lines （DR1111 ） and high susceptive lines of alfalfa （DS10） against common leaf spot were inoculated with Pseudopeziza medicaginis （Lib.） Sacc. in lab, and then the activity of PAO, DAO, CAT and SOD was measured after the inoculation of 0, 1, 2, 3, 4, 5, 10, 15 and 20 days. The results showed that the activity of 4 antioxidant enzymes in both of DR1111 and DS10 was increased at first and then decreased in different degree and at different time. The activity of DAO was higher in DR1111 than in DS10（P〈0.05） ; the activity of SOD in DS10 was higher than in DRllll after inoculation of 3d to 10d（P〈0.05）. The summit value of activity of PAO,DAO presented earlier in DR1111 than in DS10,and the summit value of activity of CAT presented later in DR1111 than in DS10.

关 键 词：紫花苜蓿 假盘菌 褐斑病 酶活性 

分 类 号：S541.1[农业科学—作物学] S432.1