HPLC法测定林芝地区丹参中丹参酮IIA含量研究  

Determination of Tanshinone IIA of Salvia Miltiorrhiza Bge.Collected from Nyingchi Prefecture by HPLC

作　　者：张小红 梁洁[2] 肖雪[2,3] 史庆龙[3] 

机构地区：[1]广东省食品药品职业技术学校,广东广州510663 [2]广东药学院,广东广州510006 [3]中山大学南沙研究院,广东广州511458

出　　处：《亚太传统医药》2015年第12期17-19,共3页Asia-Pacific Traditional Medicine

摘　　要：目的：采用高效液相色谱（HPLC）法测定不同采收期丹参不同部位丹参酮IIA的含量。方法：采用HPLC法测定，色谱柱为PhenomenexC18柱（4．60mm×250mm，5／μm）；流动相为水（A）-甲醇（B）（75：25）等度洗脱，流速为1mL／min；检测波长为270nm，柱温25℃。结果：丹参酮IIA含量测定线性范围为3．24-97．2μg／mL，R2=0．9999，平均加样回收率为99．1％。结论：不同采收期丹参不同部位丹参酮IIA含量有差异。Objective： To establish a method for Tanshinone IIA for Salvia miltiorrhiza Bge. Methods：The Phenomenex C18 analytical column （4.60minx 250ram, 5fire） was used. The mobile phases comsisted of H20 and MeOH with isoeratie elution. The flow rate was 1 mL/min,the detection wavelength was at 270 nm and the column temperature was at 25℃. Results： The method had good linear relationship within the range 3.24-97.2μg/mL of Tanshinone IIA, with the correlation coefficent higher as 0. 999 9. The average recovery was 99.1% of Tanshinone IIA. Conclusion： There were certain concentrations of Tanshinone IIA in different parts of Salvia miltiorrhiza Bge in different harvest.

关 键 词：丹参 丹参酮IIA 含量测定 

分 类 号：R284[医药卫生—中药学]