正交试验法优选补肾祛瘀颗粒的提取工艺  

Optimization of Extracting Technology for Bushen Quyu Granules by Orthogonal Test

作　　者：陈佳丽[1] 楼婷婷[1] 田文慧[1] 袁强[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《中国实验方剂学杂志》2015年第13期20-23,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省中药现代化专项(浙经信医化[2010]421号)

摘　　要：目的:优选补肾祛瘀颗粒的提取工艺,为该制剂的工业化生产提供参考。方法:采用UV测定总多糖和总黄酮含量,检测波长分别为489,510 nm;采用HPLC测定二苯乙烯苷含量,流动相乙腈-水(22∶78),检测波长320 nm。以总多糖得率为指标,通过正交试验考察料液比、提取时间、提取次数、醇沉浓度对水提工艺的影响;以总黄酮、二苯乙烯苷提取量和浸膏得率的综合评分为指标,通过正交试验考察提取时间、提取次数、乙醇用量及体积分数对醇提工艺的影响。结果:最佳水提工艺为加12倍量水浸泡1 h,回流提取3次,每次1.5 h,醇沉浓度80%;总多糖得率8.682%。最佳醇提工艺为加10倍量80%乙醇回流3次,每次1.5 h;总黄酮和二苯乙烯苷提取量分别为660.19 mg·g-1和145.91μg·g-1,浸膏得率11.81%。结论:优选的提取工艺合理可行,为补肾祛瘀方的临床推广与应用提供实验依据。Objective： To optimize extraction process of Bushen Quyu granules. Method： UV was adopted to determine contents of total polysaccharides and total flavonoids with detection wavelength at 489 nm and510 nm. HPLC was used to determine the content of stilbene glycoside with mobile phase of acetonitrile-water（ 22∶ 78） and detection wavelength at 320 nm. Taking total polysaccharides yield as index,orthogonal test was adopted to investigate effects of solid-liquid ratio,extraction time and times,alcohol precipitation concentration on water extraction process; taking comprehensive score of yields of total flavonoids,stilbene glycoside and extract as index,effects of ethanol concentration,ethanol amount,extraction time and times on alcohol extraction process were optimized by orthogonal test. Result： Optimum water extraction process was as follows： soaked 1 h and refluxing extracted thrice with 12 times the amount of water for 1. 5 h each time,alcohol precipitation concentration of 80%; yield of total polysaccharides reached 8. 682%. Optimum ethanol extraction process was： refluxing extracted thrice with 10 times the amount of 80% ethanol for 1. 5 h each time; yields of total flavonoids,stilbene glycoside and extract reached 660. 19 mg·g- 1,145. 91 μg·g- 1,11. 81%,respectively. Conclusion： These optimized extraction processes are repeatable and feasible to provide basis for industry production of Bushen Quyu granules.

关 键 词：补肾祛瘀颗粒 总多糖 总黄酮 二苯乙烯苷 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]