基于ND2基因序列的燕窝DNA条形码鉴别  

Molecular Identification of Edible Bird's Nest Based on ND2 DNA Barcode

作　　者：王凤云[1,2] 蒋颖诗 赖小平[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第13期36-40,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81173498)

摘　　要：目的:利用DNA条形码鉴定技术,快速、准确地鉴别不同种类及产地的燕窝,为该药材的质量评价提供参考。方法:提取不同产地及品种燕窝的DNA,对烟酰胺腺嘌呤二核苷酸(NADH)脱氧酶亚单位2(ND2)部分序列进行扩增和测序,利用软件MEGA6.0进行序列比对,分析变异位点,计算Kimura-2参数遗传距离,构建邻接(neighbor-joining,NJ)系统发育树。结果:NJ系统发育树显示31个官燕窝样品均与爪哇金丝燕Aerodramus fuciphagus聚为1支;1个毛燕窝样品与大金丝燕A.maximus聚为1支。但官燕窝中经细胞色素b(Cytb)鉴定生物基原为爪哇金丝燕亚种(淡腰金丝燕A.fuciphagus germani)的8个样品单独聚为1支,支持率82%,并在该段ND2序列表现为2个位点的差异,表明该段ND2序列可鉴别爪哇金丝燕的亚种。结论:ND2条形码可用于快速、准确地鉴别燕窝的生物基原。Objective： To identificate different origins and categories of commodity edible bird＇s nest （EBN） by DNA bareoding technique fastly and acurrately. Method： Total genomic DNA was isolated from different origins and categories of commodity EBN. Nicotinamide adenine dinucleotide dehydrogenase subunit 2 （ND2） gene sequences were amplified and sequenced. Identification were performed using similar Blast from C, enBank, nearest distance and neighbor-joining （NJ） methods. Result： NJ phylogenetie tree showed 31 white or silver EBN samples which were clustered with Aerodramus fuciphagus; a black EBN sample was clustered with A. maximus. However, among white or silver EBN samples, there were 8 samples clustered into a separate branch with support rate of 82% , which were identified by cytoehrome b （Cytb） gene as the subspecies （A. fuciphagus germani） , and sequence alignment showed 2 parsimony-informative sites. This result showed that segment of ND2 sequence can identify subspecies of A. fuciphagus. Conclusion： ND2 sequence is efficient barcode for identifying origin species of EBN.

关 键 词：燕窝 DNA条形码 NADH脱氧酶亚单位2 基原鉴定 

分 类 号：R282.5[医药卫生—中药学] R284.2[医药卫生—中医学]