滇重楼品质评价及其甾体皂苷类成分积累规律分析  

Quality Evaluation of Paris polyphylla var. yunnanensis and Accumulation Law Analysis of Its Steroidal Saponins

作　　者：冯丽丽[1] 张琳[1] 李海峰[1] 张成桂[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《中国实验方剂学杂志》2015年第13期41-45,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81360616);云南省教育厅基金重点项目(2013Z153)

摘　　要：目的:探讨滇重楼居群内品质差异及甾体皂苷活性成分的积累规律,为该药材的品质评价及优质种质资源筛选提供实验依据。方法:采用超高效液相色谱法(ultra performance liquid chromatography,UPLC)测定滇重楼居群内根茎、茎、叶中重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ的含量,建立各部位的UPLC指纹图谱,分析该药材居群内的品质差异及其甾体皂苷类成分的积累规律。结果:滇重楼居群内根茎和叶中总皂苷含量均>0.6%,不同部位中重楼皂苷Ⅰ,Ⅱ,Ⅶ含量具有显著性差异,而重楼皂苷Ⅵ含量无显著性差异;UPLC指纹图谱中5个共有峰为根茎、茎、叶共有,各部位共有峰UPLC指纹图谱相似度均>0.753。结论:重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ主要在植物的叶绿体中合成并进行储藏和分配,经茎向下运输,主要在根茎和叶中积累和储存,总皂苷含量在不同部位的积累规律为叶>根茎>茎;滇重楼不同部位中主要活性成分含量及种类差异较大。Objective： To investigate quality differences and accumulation law of steroidal saponins within Paris polyphylla var. yunnanensis,to provide a basis for quality evaluation and quality germplasm screening of the medicinal material. Method： UPLC was employed to determine contents of polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶfrom roots, stems and leaves in the same population of P. polyphylla var. yunnanensis. UPLC fingerprint of different parts was established,quality differences of P. polyphylla var. yunnanensis and its accumulation law of steroidal saponins were analyzed. Result： Contents of total saponins from roots and leaves in the same population of P. polyphylla var. yunnanensis were more than 0. 6%. There were significant differences about contents of polyphyllin Ⅰ,Ⅱ and Ⅶ,but polyphyllin Ⅵ showed no significant difference. Five common peaks of UPLC fingerprint were selected in roots, stems and leaves, similarity of characteristic peaks were above0. 753. Conclusion： Polyphyllin Ⅰ,Ⅱ,Ⅵ and Ⅶ are synthesized,stored and distributed in the chloroplasts of green plants, then transported down by stems and majorly accumulated in leaves and rhizomes at last,accumulation law of total saponins content in different parts is leaves〉roots〉stems. There are large differences about contents and species of main active ingredients among roots, stems and leaves of P. polyphylla var.yunnanensis.

关 键 词：滇重楼 总皂苷 品质评价 活性成分积累 重楼皂苷 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]