苍术提取物对脾虚证大鼠胃黏膜结构及胃肠功能的影响  

Effect of Atractylodis Rhizoma Extract on Structure of Gastric Mucosa and Gastrointestinal Function in Rats with Spleen Deficiency

作　　者：刘芬[1,2] 刘艳菊[1] 田春漫[2] 

机构地区：[1]湖北中医药大学,武汉430065 [2]湖北民族学院,湖北恩施445000

出　　处：《中国实验方剂学杂志》2015年第13期100-104,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073051);国家中管局中医药行业科研专项(201007012-2)

摘　　要：目的:探讨苍术提取物保护脾虚大鼠胃黏膜及胃肠功能的作用,阐明苍术提取物干预脾虚证的作用机制。方法:采用喂饲小承气汤煎剂加饥饱失常建立大鼠脾虚证模型,然后将动物随机分为模型组、苍术提取物高、中、低剂量组(20,10,5 g·kg-1)、多潘立酮组(5 mg·kg-1),连续ig给药10 d。采用碳末ig法行大鼠胃内残留率、小肠推进比测定,HE染色观察大鼠胃黏膜病理学,透射电镜观察腺胃区胃黏膜组织细胞及细胞间连接的超微结构改变,激光多普勒微循环血流计测定大鼠胃黏膜血流量,免疫组化法测定大鼠胃黏膜组织中三叶因子1(TFF1)及结肠组织中受体酪氨酸蛋白激酶(c-kit)的表达量。结果:与正常组比较,模型组大鼠胃黏膜形态学及超微结构明显异常,胃黏膜血流量及胃黏膜组织中TFF1和结肠组织中c-kit的表达量明显降低(P<0.01);胃内残留率明显升高,小肠推进比明显降低(P<0.01),经苍术提取物干预后各指标明显改善(P<0.01,P<0.05)。结论:苍术提取物可抑制脾虚证大鼠胃黏膜损害,保护和修复损伤的黏膜组织,并改善脾虚证大鼠的胃肠功能。Objective： To observe the role of Atractylodis Rhizoma extract on protecting gastric mucosa and gastrointestinal dysfunction of rats with spleen deficiency syndrome, and to elucidate the mechanism of Atractylodis Rhizoma extract regulating spleen deficiency syndrome. Method： Male rats were fed with Xiaochengqi decoction and hunger to establish spleen deficiency syndrome models. Then the animal were randomly divided into model group,Atractylodes Rhizome extract high,middle and low dose group（ 20,10,5 g·kg- 1）,Domperidone group 5 mg·kg- 1. After 10 days of treatment,charcoal gavage method was used o determine residual rate of stomach and small intestinal propulsion ratio of the rats,hematoxylin and eosin（ HE） staining was used to observe the gastric mucosa pathological and histological changes,like gastric mucosa tissue cells of glandular stomach area and ultrastructural alterations of intercellular junctions with transmission electron microscopy method,laser Doppler blood flow meter was used to measure gastric mucosal blood flow,immunohistochemical was used to determinate the expression of trefoil factor 1（ TFF1） in gastric mucosa and c-kit expression in colon tissue volume. Result：Compared with the normal group,gastric mucosal morphology had significantly abnormal change in model group;gastric mucosal blood flow,gastric mucosa TFF1 and colon tissue volume c-kit expression were decreased（ P〈0. 01）; residual rate of stomach was increased and small intestinal propulsion ratio was decreased（ P〈0. 05,P〈0. 01）. After intervention of Atractylodis Rhizoma extract, those indicators were improved（ P〈0. 05, P〈0. 01）. Conclusion： Atractylodis Rhizoma extract can inhibit the gastric mucosal damage of spleen deficiency rat,protect and repair the damaged mucous membrane organization,and improve the gastrointestinal function of spleen deficiency rats.

关 键 词：苍术提取物 脾虚证 胃肠功能 信号传导 

分 类 号：R285.5[医药卫生—中药学]