舒郁胶囊对PMS肝气郁证模型大鼠下丘脑μ-阿片受体的影响  

Effect of Shuyu Capsule on Expression of μ-opioid Receptors in Hypothalamus of Premenstrual Syndrome of Liver-Qi Stagnation Rats

作　　者：王杰琼[1] 许莉莉[1] 张丽娜[2] 薛玲[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,济南250355 [2]威海市立医院,山东威海264200

出　　处：《中国实验方剂学杂志》2015年第13期105-108,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81173162)

摘　　要：目的:通过观察经前期综合征(PMS)肝气郁证模型大鼠下丘脑中μ-阿片受体(MOR)的蛋白表达,探讨舒郁胶囊治疗PMS肝气郁证的机制。方法:Wistar大鼠56只,随机分为正常组、模型组、舒郁胶囊组(0.408 g·kg-1)和纳洛酮组(0.4mg·kg-1),每组14只,旷场实验和阴道涂片筛选动情周期规律非接受期大鼠进入实验,除正常组,其余各组采用慢性束缚应激法复制PMS肝气郁证模型,旷场实验对模型进行行为学评价,各组大鼠下丘脑采用免疫荧光化学技术(IF)和Western blot分别观察MOR分布及蛋白表达变化。结果:宏观行为学表明,与正常组比较,模型组大鼠旷场总分显著降低(P<0.01);免疫荧光结果显示,与正常组比较,模型组大鼠下丘脑细胞分布排列杂乱且增多,与模型组比较,舒郁胶囊、纳洛酮给药组均得到显著改善;蛋白免疫印迹结果显示,与正常组比较,模型组下丘脑中MOR表达水平显著升高(P<0.01),与模型组比较,舒郁胶囊、纳洛酮给药组MOR表达水平均显著下降(P<0.01)。结论:舒郁胶囊可通过对下丘脑中MOR的拮抗作用而改善PMS肝气郁证的症状,另外,下丘脑中MOR上调可能是PMS肝气郁证的发病机制之一。Objective： To observe the effect of Shuyu capsule on μ-opioid receptors（ MOR） expression in the hypothalamus of premenstrual syndrome（ PMS） with liver-qi stagnation model rats,and to investigate its possible mechanism. Method： Rats were selected by using an open field test and vaginal smear in this experiment. The selected rats were divided into 4 groups： the normal group,the model group,the Shuyu capsule group（ 0. 408 g·kg- 1） and the naloxone group（ 0. 4 mg·kg- 1）. The PMS with liver-qi stagnation model in rats was prepared by a chronic bondage stress method. The distribution and protein level of MOR in hypothalamus of rats were observed by immunofluorescence chemical technology and Western blot. Result： Compared with the normal group,the scores of open field test decreased,the distribution of messy-arranged MOR in hypothalamus increased,MOR protein expression increased in the model group（ P〈0. 01）. Compared with the model group,the scores of open field test increased,the distribution of messy-arranged MOR in hypothalamus decreased,MOR protein expression decreased in the Shuyu capsule and naloxone groups（ P〈0. 01）. Conclusion： Shuyu capsule could improve the symptoms of PMS with liver-qi depression syndrome,which may be achieved by regulating the MOR in the hypothalamus. In addition,the up-regulation of MOR in the central nervous system may be one of the pathophysiologic mechanisms.

关 键 词：舒郁胶囊 PMS肝气郁证 Μ-阿片受体 下丘脑 

分 类 号：R285.5[医药卫生—中药学]