白花丹醌对TGF-β_1刺激人肝星状细胞α-SMA表达的影响  

Effect of Plumbagin on α-SMA Expressions in Human Hepatic Stellate Cells Stimulated by TGF-β_1

作　　者：杨成芳[1] 李勇文[1] 钟毓娟[1] 李丽[1] 熊美丽[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中国实验方剂学杂志》2015年第13期114-117,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西高校科学技术研究项目(KY2015LX280);桂林医学院高层次人才科研启动基金项目(KY2012087)

摘　　要：目的:研究白花丹醌(plumbagin)对转化生长因子-β1(TGF-β1)刺激的体外培养人肝星状细胞HSC-LX2α-平滑肌肌动蛋白(α-SMA)mRNA和蛋白表达的影响。方法:体外培养HSC-LX2,随机设立空白组,TGF-β1刺激的模型组,TGF-β1+白花丹醌2.0μmol·L-1高剂量组,TGF-β1+白花丹醌1.5μmol·L-1中剂量组和TGF-β1+白花丹醌1.0μmol·L-1低剂量组,各药物与细胞孵育72 h后,采用RT-PCR检测各组HSC-LX2α-SMA mRNA的表达,采用免疫细胞化学方法(ICC)检测HSCLX2中α-SMA蛋白的表达。结果:与正常组比较,模型组α-SMA mRNA及蛋白表达明显升高(P<0.01);与模型组比较,白花丹醌作用72 h后,高、中剂量组HSC-LX2细胞中α-SMA mRNA的表达明显降低(P<0.01);免疫细胞化学结果显示白花丹醌高、中剂量组对HSC-LX2细胞α-SMA蛋白的表达有显著地抑制作用(P<0.05),尤以高剂量组更明显(P<0.01)。结论:白花丹醌能抑制HSC-LX2的活化和增殖,其机制之一可能是从mRNA水平和蛋白水平抑制α-SMA的表达,从而发挥其抗肝纤维化作用。Objective： To observe the effect of plumbagin on the mRNA and protein expressions ofα-smooth muscle actin（ α-SMA） in human hepatic stellate cell（ HSC）-LX2 cells stimulated by transforming growth factor-β1（ TGF-β1） in vitro. Method： HSC-LX2 cells were cultured in vitro and divided into the blank group,the model group,the high-,medium-and low-dose plumbagin groups（ 2. 0,1. 5,1. 0 μmol ·L- 1）. After being incubated with each drug for 72 hours,the mRNA expression of α-SMA was assayed by RT-PCR and the protein expression of α-SMA was assayed by immunocytochemistry. Result： Compared with blank group,the mRNA and protein expressions of α-SMA in HSC-LX2 cells increased in the model group（ P〈0. 01）. Compared with model group,the mRNA and protein expressions of α-SMA were inhibited in the high-and medium-dose plumbagin groups（ P〈0. 05,P〈0. 01）. Meanwhile,there were better results in the high-dose plumbagin group（ P〈0. 01）.Conclusion： Plumbagin could inhibit the activation and proliferation of HSC-LX2 cells and have certain antihepatic fibrosis effect. The mechanism may be related to restraining the mRNA and protein expressions of α-SMA.

关 键 词：白花丹醌 转化生长因子-β1 肝星状细胞系-LX2 Α-平滑肌肌动蛋白 抗肝纤维化 

分 类 号：R285.5[医药卫生—中药学]