托盘根水煎剂降糖作用分析  

Anti-hyperglycemic Effect of Rubus crataegiflolius Roots Decoction

作　　者：雷钧涛[1] 莫绍凌 陈爽[1] 王杰[1] 

机构地区：[1]吉林医药学院,吉林吉林132013 [2]吉林工贸学校,吉林吉林132013

出　　处：《中国实验方剂学杂志》2015年第13期118-121,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省教育厅"十二五"科学技术研究基金项目(吉教科合字[2014]第362号);吉林省科技发展计划自然科学基金项目(20140101142JC)

摘　　要：目的:探讨托盘根水煎剂对四氧嘧啶诱导的糖尿病大鼠的降糖作用。方法:Wistar大鼠80只,采用大鼠尾静脉注射四氧嘧啶复制糖尿病大鼠模型。随机分为模型组(ig 13.5 m L·kg-1的蒸馏水),阳性药组(210 mg·kg-1的盐酸二甲双胍水溶液),托盘根低、高剂量组(分别ig 1.35,2.70 g·kg-1托盘根水煎剂),另设正常组,每组10只,每天ig 1次,共13 d。检测空腹血糖(FBG),一氧化氮(NO),丙二醛(MDA),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)及脏器系数的影响。结果:与正常组比较,模型组FBG,SOD,GSH-Px水平明显升高,NO,MDA含量明显降低(P<0.05);经托盘根治疗后,治疗组FBG水平较模型组降低(P<0.05),低剂量组血清中NO和高剂量组血清中MDA含量较模型组升高(P<0.05),而高剂量组血清中SOD和低、高剂量组血清中GSH-Px含量较模型组降低(P<0.05),且托盘根水煎剂对所取脏器均有修复作用。结论:托盘根水煎剂可通过增强机体的抗氧化作用,促进胰岛功能恢复而发挥降血糖作用。Objective： To observe the anti-hyperglycemic effect of the decoction of Rubus crataegiflolius roots in diabetic rats induced by alloxan. Method： Diabetic rat models were copied by iv alloxan through tail vein,then rats were randomly divided into 4 groups; the model group（ distilled water,13. 5 m L·kg- 1）,the positive control group（ metformin hydrochloride,210 mg ·kg- 1）,the low and high dose groups（ decoction of R.crataegifloliums roots,1. 35,2. 70 g·kg- 1） of 10 rats each. Another 10 healthy rats were assigned to the normal group（ distilled water,13. 5 m L·kg- 1）. The corresponding medicines were intragastrically administrated to rats once daily for 13 days. Changes of fasting blood-glucose（ FBG）,nitrogen oxide（ NO）,malonaldehyde（ MDA）,superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px） were detected. The organ coefficient was calculated. Result： Compared with the normal group,the levels of FBG,SOD and GSH-Px increased,the levels of NO and MDA decreased in the model group（ P〈0. 05）. Compared with model group,the levels of FBG and GSH-Px decreased in the low and high dose groups（ P〈0. 05）,the NO level increased in the low dose group（ P〈0. 05）,the MDA levels increased in the high dose group（ P〈0. 05）,and the SOD level decreased in the high dose group（ P〈0. 05）. Moreover,the organ damage could be repaired by R. crataegifldiums roots decoction.Conclusion： R. crataegiflolius roots decoction could decrease blood glucose by improving the anti-oxidation function of the body and the functional recovery of the isle.

关 键 词：托盘根水煎剂 四氧嘧啶 糖尿病 一氧化氮 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]