淫羊藿苷对AD模型大鼠神经元超微结构的影响  

Influence of Icariin on Ultrastructural Changes of Hippocampus in Alzheimer Disease Rat

作　　者：郭力军 计德利 孙计桃[3] 李自如 

机构地区：[1]内蒙古自治区人民医院神经内科,内蒙古呼和浩特010017 [2]赤峰市医院神经内科,内蒙古赤峰024099 [3]内蒙古医科大学基础医学院,内蒙古呼和浩特010059

出　　处：《内蒙古医学杂志》2015年第6期657-660,F0002,共5页Inner Mongolia Medical Journal

基　　金：内蒙古自治区科技攻关项目(20070501)

摘　　要：目的研究淫羊藿苷对阿尔茨海默病(Alzheimer disease,AD)模型大鼠行为学及海马神经元超微结构的影响,探讨淫羊藿苷治疗AD的作用机理。方法经水迷宫筛选的18只雄性Wistar大鼠随机分为正常对照组,模型组,淫羊藿苷治疗组。模型组、淫羊藿苷治疗组大鼠给予腹腔注射脂多糖(LPS)制作实验性AD炎症模型,采用Morris水迷宫实验观察大鼠学习记忆能力,实验结束后开颅取脑,电镜下观察各组大鼠脑组织海马神经元超微结构的变化。结果定位航行实验中,记录各组大鼠逃避潜伏期,模型组与正常对照组大鼠比较差异有统计学意义(P<0.05),治疗组与模型组大鼠比较差异有统计学意义(P<0.05);空间探索实验中,记录各组大鼠靶象限活动时间百分比,模型组与正常对照组大鼠比较差异有统计学意义(P<0.05),治疗组与模型组大鼠比较差异有统计学意义(P<0.05)。对照组海马神经元超微结构正常,与对照组比较,模型组大鼠海马神经元细胞胞膜节段性断裂、不清,核染色质聚集成块、边集;线粒体肿胀,内有空泡形成;高尔基体肿胀;内质网扩张;淫羊藿苷治疗组大鼠神经细胞上述病理变化有所减轻。结论经Morris水迷宫行为学检测,腹腔注射LPS制作AD炎症模型成功;淫羊藿苷能够改善AD模型大鼠的学习记忆能力,对模型大鼠海马神经元超微结构损伤具有保护作用。Objective To observe the influence of icariin on ultrastructural changes of hippocampus in Alzheimer disease rat and explore the mechanism o f icariin to AD. Methods After the Morris water maze test, 18 Wistar rats were randomly divided into three groups： normal control group, model group, Icariin treatment group. The i.P. Injection of LPS for rats of model group and Icariin treatment group were administrated, Morris water maze test was used to evaluate the memory dysfunction and electron microscope was used to observe the ultrastructure of hippocampus neuron. Results In Water maze test , data of the escape latency of rats in each group was recorded, there was a significant difference between the model group and the normal control group（P 〈0.05）, as well as the same result between the treatment group and the model group（P 〈0. 05）;In spatial probe test, average time percentage spent on the target quadrant of model group was significantly decreased（P 〈0.05）while the time were significantly increased of treatment group than those of model group（P 〈 0.05）; In the model group, the cellular membrane was irregular and discontinue, nuclear membrane was cloudinessl. The nuclear chromatin was aggregate. The mitochondria was oncootic and vacuolate. The golgi apparatus was oncootic;The endoplasmic reticulum was dilative. These ultrastructural pathological changes in the treatment group were not apparent. Conclusion In this experiment, AD model mice which administrated LPS appeared decrease in memory dysfunction , it indicated that a successful AD rat model has been made. Icariin is beneficial for improving the memory dysfunction, attenuating the neural damage of AD model rat.

关 键 词：阿尔茨海默病 淫羊藿苷 行为学 超微结构 

分 类 号：R592[医药卫生—老年医学]