生命早期不同营养状态对大鼠体质量及下丘脑mTOR表达的影响  

Effect of different nutritional status during early life on the weight and hypothalamic m TOR expression in rats

作　　者：余婧[1,2] 朱顺叶[1] 唐本玉[1] 潘思年[1] 张敏[1] 彭碧秀[1] 

机构地区：[1]中山大学附属第三医院儿科,广州510630 [2]中山大学中山医学院

出　　处：《新医学》2015年第6期363-368,共6页Journal of New Medicine

基　　金：广东省自然科学基金(94510089010021202)

摘　　要：目的研究生命早期的营养状态对各生长期大鼠体质量、下丘脑哺乳动物雷帕霉素靶蛋白(m TOR)表达的影响。方法将84只1日龄SD大鼠分为小窝组(40只)与对照组(44只,哺乳期死亡2只),母乳喂养至21日龄。断乳后将2组大鼠各自随机分为高脂组及普通组,于不同生长期取鼠测量体质量、Lee's指数、内脏脂肪质量(VFM)及检测下丘脑m TOR的表达情况。结果未发育期小窝组体质量明显高于对照组(P<0.05)。自发育前期起,高脂喂养雌性大鼠Lee's指数及VFM均高于普通喂养大鼠,高脂喂养的小窝大鼠的体质量明显高于普通喂养的对照大鼠(P均<0.05)。高脂喂养雄性大鼠体质量、Lee's指数及VFM均高于普通喂养大鼠(P<0.05)。小窝组下丘脑m TOR的相对表达量高于对照组(P=0.015),高脂喂养大鼠下丘脑m TOR相对表达量高于普通喂养大鼠(P<0.05)。结论生命早期不同营养状态对大鼠不同生长时期的体质量有影响,并持续影响其下丘脑m TOR的表达,最终可能导致能量代谢紊乱。Objective To evaluate the effect of different nutritional status during early life upon the weight and the expression of hypothalamic mammalian target of rapamycin （mTOR）in rats of varying develop-mental stages.Methods Eighty-four Sprague-Dawley rats aged 1 day were assigned into the small （n =40） and control groups （n =44,2 rats died during lactation period）,and breast-fed until 21 days of age.Then, the rats were randomly divided into the high-fat feeding and normal feeding groups.The rats＇weight,Lee＇s in-dex,visceral fat mass （VFM）and the expression of hypothalamic mTOR were measured at different develop-mental stages.Results The weight of prepubertal rats in the small group was significantly higher than that in the control group （P 〈0.05）.Since early pubertal stage,the Lee＇s index and VFMof female rats in the high-fat feeding group were considerably higher than those in the normal feeding group （both P 〈0.05）.The weight of rats fed with high-fat diet in the small group was significantly higher compared with that of control rats with normal feeding （P 〈0.05）.The weight,Lee＇s index and VFMof male rats with high-fat feeding were signifi-cantly higher compared with those of the control rats （all P 〈0.05）.The relative expression of hypothalamic mTOR of the rats in the small group was dramatically higher than that of the animals in the control group （P =0.01 5）.The relative expression of hypothalamic mTOR in the rats with high-fat feeding was significantly higher than that in rats with normal feeding （P 〈0.05）.Conclusions The nutritional status during early life exerts an effect upon the rat weight during different developmental stages and persistently affects the expression of hy-pothalamic mTOR,probably eventually leading to energy metabolism disorders.

关 键 词：生命早期 营养 体质量 哺乳动物雷帕霉素靶蛋白 大鼠 

分 类 号：R723.14[医药卫生—儿科]