青壮年军人腰腿痛患者HLA-B27及其基因亚型检测分析  

Detection of HLA-B27 and genetic subtypes in young soldiers with low back pain

作　　者：高文博[1] 徐海栋[2] 刘刚[2] 李卫巍[1] 夏欣一[1] 虞伟[1] 李晓军[1] 

机构地区：[1]南京军区南京总医院(南方医科大学南京临床医学院)临床中心实验科,210002 [2]南京军区南京总医院(南方医科大学南京临床医学院)骨科,210002

出　　处：《东南国防医药》2015年第3期233-235,共3页Military Medical Journal of Southeast China

基　　金：国家自然科学基金项目(81470071)

摘　　要：目的通过检测2126例青壮年军人腰腿痛患者人类白细胞抗原B27(HLA-B27)阳性率和表达强度,了解本地区军人强直性脊柱炎(ankylosing spondylitis,AS)与HLA-B27抗原相关性,并初步探讨其对AS的临床诊断意义;通过检测确诊AS军人患者HLA-B27基因亚型,了解本地区军人HLA-B27基因亚型分布情况。方法用流式细胞仪分析2013年8月1日-2014年1月10日2126例因腰腿痛入南京军区南京总医院就诊的军人患者HLA-B27抗原表达情况;用荧光定量PCR法对其中确诊AS患者HLA-B27基因亚型进行检测分析。结果 2126例患者中HLA-B27阳性者158例,阳性率为7.4%,男女患者HLA-B27抗原阳性率分别为7.1%和10.0%(P<0.01);33例HLA-B27阳性确诊患者中,B*2704和为B*2705为主要型别,分别占51.5%和42.4%。结论 HLA-B27对于AS仅有辅助诊断作用,不宜作为AS的常规筛选指标。Objective To investigate the relationship and diagnostic significance between ankylosing spondylitis and HLA- B27 antigen in soldiers in one military district by detecting the positive rate and expression intensity of HLA-B27 antigen in 2126 young soldiers with low back pain, and evaluate its clinical significance. To investigate the distribution of genetic subtypes of AS by detecting genetic subtypes of HLA-B27 in confirmed soldiers. Methods The expression of HLA-B27 antigen of peripheral blood T lymphocytes in 2126 hospital admission patients was analyzed by flow cytometry. Genetic subtypes of HLA-B27 was determined by fluorescence quantitative polymerase chain reaction assay. Results 158 patients were positive in 2126 patients. The positive rate of antigen HLA- B27 was 7.4%, and the male and female patients＂ positive rates of HLA-B27 antigen were 7. 1% and 10.0%, respectively （P 〈 0.01 ）. Among the 33 confirmed AS patients with HLA-B27 antigen positivity, B ＊ 2704 and B ＊ 2705 were the main type with account- ing for 51.5% and 42.4% respectively. Conclusion HLA-B27 is helpful for auxiliary diagnosis of AS, but is not suitable as a screening indictor of AS.

关 键 词：人类白细胞抗原B27 流式细胞术 强直性脊柱炎 军人 基因分型 

分 类 号：R593.23[医药卫生—内科学]