斜带石斑鱼(♀)×鞍带石斑鱼(♂)杂交子代幼鱼消化道粘液细胞和胃泌素细胞的研究  

Study on mucous and gastrin cells of digestive tract in Qinglong grouper( Epinephelus coioides♀ × E. lanceolatus♂) juveniles

作　　者：李加儿[1] 吴水清[1,2] 区又君[1] 刘江华[1,2] 温久福[1] 王鹏飞[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《南方水产科学》2015年第3期67-73,共7页South China Fisheries Science

基　　金：广东省科技计划项目(2012A020602016);广东省海洋渔业科技推广专项(B201101B04);中国水产科学研究院基本科研业务费专项(2012A 0703);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2012TS30,2012TS31)

摘　　要：利用组织化学(AB-PAS)和免疫组织化学(SABC)的方法分别对斜带石斑鱼(Epinephelus coioides♀)×鞍带石斑鱼(E.lanceolatus♂)杂交子代(青龙斑)幼鱼消化道的粘液细胞和胃泌素(gastrin,Gas)分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有4种类型的粘液细胞。肠道粘液细胞数量为中肠>后肠>前肠。利用免疫学的方法研究青龙斑幼鱼消化道Gas细胞的分布,表明幼鱼的整个肠道和幽门盲囊均有Gas细胞的存在,食道和胃中未发现Gas免疫阳性细胞。We studied the distribution of mucous and gastrin cells of digestive tract in Qinglong grouper （ Epinephelus coioides ♀ x E. lanceolatus ♂ ） juveniles by histochemistry （AB-PAS） and immunohistochemistry （SABC）. Mucous cells in esophagus of juvenile were of Type Ⅰ , Type Ⅱ , Type Ⅲ and Type Ⅳcontaining neutral and acidic mucoitin; mucous cells of Type Ⅰ , Type Ⅱand Type Ⅲ were found in cardiac stomach; mucous cells of Type Ⅰ , Type Ⅲ and Type IV were found in fundic stomach. Many mucous cells of Type Ⅰ , and Type Ⅳ could be found around gastric gland, and only mucous cells of Type Ⅰ containing neutral mucointin was found in pyloric stomach. Pyloric caeca and intestines contained neutral and acidic mucointin. Mucous cells in the pyloric caeca were mostly of Type Ⅱ but less Type Ⅲ. Four types of mucous cells could all be observed in foregut, midgut and hindgut. The descending order of number of intestinal mucous cells was midgut 〉 hindgut 〉 foregut. Moreover, the study on the distribution of gastrin cells in the diges- tive tract by immunohistochemistry indicates that the gastrin cells were found in entire intestine and pyloric caeca but not in esophagus and stomach.

关 键 词：斜带石斑鱼 鞍带石斑鱼 杂交子代 消化道 粘液细胞 胃泌素细胞 

分 类 号：S917.4[农业科学—水产科学]