支气管肺泡灌洗液中β-联蛋白、转化生长因子β_1、Clara细胞分泌蛋白16、Ⅱ型肺泡细胞表面抗原6在支气管肺发育不良中的表达及意义  

The expression and significance of β-Catenin,transforming growth factor beta 1 (TGF-beta 1),Clara cell secretory protein 16 (cc16) and surface antigen 6 of alveolar epithelial cell Ⅱ(KL-6) in bronchialveolar lavage fluid(BALF)of children with bronchial p

作　　者：杨敏[1] 李林瑞[1] 黄建宝[1] 孟燕妮[1] 陈艳萍[1] 

机构地区：[1]湖南省儿童医院呼吸内二科,长沙410011

出　　处：《中国中西医结合儿科学》2015年第3期219-223,共5页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：湖南省科技计划项目(2012SK3256)

摘　　要：目的探讨β-联蛋白、转化生长因子β1(TGF-β1)、Clara细胞分泌蛋白16(cc16)、Ⅱ型肺泡细胞表面抗原6(KL-6)在支气管肺发育不良(BPD)患儿支气管肺泡灌洗液(BALF)中的表达及其意义。方法机械通气早产儿70例,其中40例诊断为BPD的患儿作为观察组,30例非BPD患儿作为对照组。采用酶联免疫吸附试验法检测两组患儿机械通气1、24、48、72hBALF中β-联蛋白、TGF-β1、cc16、KL-6水平。结果观察组平均胎龄及出生体质量显著低于对照组,差异有统计学意义(P<0.05),吸氧时间及机械通气时间显著长于对照组,差异有统计学意义(P<0.01)。随机械通气时间的延长,观察组cc16水平呈逐渐降低趋势,而β-联蛋白、TGF-β1、KL-6水平呈逐渐升高趋势,且48、72h时均与1h时各指标水平差异有统计学意义(P<0.01)。对照组TGF-β1、cc16于机械通气72h时与1h时水平差异有统计学意义(P<0.01),而β-联蛋白、KL-6水平在各时间点的差异均无统计学意义(P>0.05)。观察组机械通气各时间点β-联蛋白、TGF-β1水平均显著高于对照组各相应时间点指标水平,cc16显著低于对照组水平,差异有统计学意义(P<0.05),而KL-6在机械通气48、72h时高于对照组相应时间点,差异有统计学意义(P<0.05),余时间点差异无统计学意义(P>0.05)。各时间点比较,BPD中重度组β-联蛋白、TGF-β1、KL-6水平均显著高于轻度组,差异有统计学意义(P<0.05),而cc16水平显著低于轻度组,差异有统计学意义(P<0.05)。多元逐步回归分析结果显示,机械通气时间、吸氧时间的延长及低cc16水平是BPD发生的危险因素(P<0.01)。结论机械通气早产儿BPD的发生与机械通气、吸氧时间的延长及低cc16水平密切相关,通过监测早产儿初始机械通气BALF中β-联蛋白、TGF-β1、cc16水平可对早产儿BPD的发生提供预测依据。Objective To investigate the expression ofβ-Catenin,transforming growth factor beta 1（TGF-beta 1）,Clara cell secretory protein 16（cc16）,and surface antigen 6of alveolar epithelial cell Ⅱ（KL-6）in bronchoalveolar lavage fluid（BALF）of children with bronchial pulmonary dysplasia（BPD）and its significance.Method Of 70 cases of premature infants with mechanical ventilation,40 cases diagnosed with BPD were included in observation group,30 cases of non-BPD patients were as control group.Detect the levels ofβ-Catenin,TGF-beta 1,cc16 and KL-6in BALF of two groups of children respectively after 1,24,48 and 72hof mechanical ventilation using enzyme-linked immunosorbent assay method.Result Average gestational age and birth body mass of observation group were significantly lower than those of the control group,the difference being statistically significant（P〈0.05）.The time of mechanical ventilation and oxygen therapy were significantly longer thanthe control group,the difference being statistically significant（P〈0.01）.With longer duration of mechanical ventilation,the levels of cc16 of observation group showed a downward trend,andβ-Catenin,TGF-beta 1and KL-6levels showed an upward trend,and the level difference of each index in time point of 48 and 72hwas statistically significant compared with that of 1h（P〈0.01）.The difference of the level of TGF-beta 1and cc16 of control group at time point 1hand 72 hof mechanical ventilation was statistically significant（P〈0.01）,but the differences of level of beta protein and KL-6at various time points had no statistical significance（P〉0.05）.Index levels of β-Catenin and TGF-beta 1of observation group with mechanical ventilation at each time point were significantly higher than control group in the corresponding time point,but cc16 was significantly lower than the control group,the difference being statistically significant（P〈0.05）,while the KL-6of observation group was higher than the control group in the corresponding time poin

关 键 词：支气管肺发育不良 β-联蛋白 转化生长因子Β1 Clara细胞分泌蛋白16 Ⅱ型肺泡细胞表面抗原6 婴儿 新生 

分 类 号：R563.12[医药卫生—呼吸系统]