玉米氰化物解毒基因β-CAS和NIT在非生物胁迫下的表达与生物学效应  

Expression and Biologic Effect of Cyanide Detoxification Genes β-CAS and NIT in Maize Under the Antibiotic Stress

作　　者：季静[1,2] 柳洁[1,2] 王罡[2] 杜希龙[1,2] 曹海燕[1,2] 荣非 金超[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津大学遗传工程研究所,天津300072

出　　处：《天津大学学报（自然科学与工程技术版）》2015年第5期409-414,共6页Journal of Tianjin University：Science and Technology

基　　金：国家自然科学基金资助项目(31271793;31271419);国家转基因生物新品种培育科技重大专项资助项目(2014ZX08003-002B)

摘　　要：氰化物(cyanide)对生物有机体和环境都有剧毒.在非生物胁迫条件下,植物细胞内会产生大量的氰化物,这些氰化物对细胞具有毒害作用,基因β-CAS和NIT是玉米细胞氰化物解毒途径上的重要基因.本研究分别以200,mmol/L Na Cl胁迫条件下第2,h、8,h、24,h和48,h的天塔5号(F1)及其亲本三叶期的幼苗作为实验材料,对其光合速率、生长势、叶片氰化物含量及氰化物解毒途径重要相关基因β-CAS和NIT的表达量进行了检测和统计学分析.研究结果表明,F1代的根长显著长于亲本;在盐胁迫处理下,三系叶片的光合速率均发生下调,其中F1的光合速率和母本没有显著差异,但是显著高于父本;F1叶片的氰化物含量比母本和父本更早发生下降;三系的基因β-CAS表达量均上调,上调幅度从大到小的顺序依次是母本、F1和父本;F1和母本的NIT基因表达量下调,父本则发生上调.Cyanide is poisonous to live organisms and environment. The cyanide content would burst out in cells when the plants are under the antibiotic stress, which is harmful to plants. Genes β-CAS and NIT are important genes involved in the cyanide detoxication. The Tianta 5 （F1） and its parent lines were treated with 200 mmol/L NaCI for 2 h, 8 h, 24 h and 48 h, and the same lines were used as the control group when they were at three-leaf stage. The photosynthetic rate, the plant character, the cyanide content and the gene expression of β-CAS and NIT of Tianta 5 and its parent lines were detected. Then the data were analyzed statistically. The results indicated that the root of F1 was significantly longer than those of the parent lines; the photosynthetic rates of all lines were down-regulated after treated by salt, among which no significant difference was found between F 1 and female parent. However, the pho- tosynthetic rate of the male parent is significantly lower than that of F1. The cyanide content of F1 decreased earlier than its parents and the expression level of β-CAS was up-regulated for all lines, the female parent increasing the most while the male parent the least. The expression level of NIT of F 1 and female parent was down-regulated while that of the male parent was up-regulated.

关 键 词：玉米 氰化物 实时定量PCR 盐胁迫 非生物胁迫 

分 类 号：Q786[生物学—分子生物学]