慢性乙型肝炎患者肝组织中黑色素瘤缺乏因子2表达与病变程度的相关性  

Expression of absent in melanoma 2 in the liver and correlation with the degrees of liver damage in patients with chronic hepatitis B

作　　者：庞秀青 李新华[1] 莫志硕[1] 冯智英[2] 潘宇航[2] 雷姿颖[1] 郑杏容 高志良[1] 谢冬英[1] 

机构地区：[1]中山大学附属第三医院感染科,热带病防治研究教育部重点实验室,广东省肝脏疾病研究重点实验室,广州市2510630 [2]中山大学附属第三医院病理科,广州市2510630

出　　处：《中华实验和临床感染病杂志（电子版）》2015年第3期27-30,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金："十二五"国家科技重大专项(No.2012ZX09303001;No.2012ZX10002007;No.2012ZX10002004)

摘　　要：目的初步探讨DNA识别受体之一黑色素瘤缺乏因子2（AIM-2）在慢性乙型肝炎患者肝脏组织中表达分布及其与肝组织病变程度的相关性。方法利用免疫组织化学方法对54例CHB患者肝组织活检标本、19例慢加急性肝功能衰竭（HBV：AcLF）肝移植术后病肝组织标本及20例健康供体肝脏组织标本进行AIM-2检测，利用Image—Pro-Plus6．0软件测定AIM-2积分吸光度值。肝脏组织炎症程度分为G1～4和纤维化程度分为s0～4，并进行HAl评分。两组问比较采用独立样本的秩和检验，多组间比较用Kruskal．wallis检验分析，各组数据间相关性分析采用Spearman相关分析。结果AIM．2表达定位于肝实质细胞的胞质内。正常肝组织中极少表达，HBV相关患肝组织中表达显著增多。患者肝脏组织AIM-2积分吸光度值中位数在HBV-ACLF组为19772．48，显著高于CHB组的4996．88（z=5．008，P〈0．001）；CHB组显著高于健康对照组的2296．49（Z=-3．028，P=0．002）；患肝组织AIM-2表达水平与肝脏炎症HAl评分正相关（r=0．707，P〈0．0001）。结论AIM-2在正常肝组织极少表达；但在慢性乙型肝炎患者肝组织表达显著增加，且随肝脏炎症的加重而表达增强，提示AIM-2可能在慢性乙型肝炎肝脏损伤机制中发挥重要作用。Objective To investigate the expression of absent in melanoma 2 （AIM-2） in liver of patients with chronic hepatitis B, and to evaluate the correlation between the expression level of AIM- 2 and the degrees of liver damage. Methods AIM2 were detected in 73 liver specimens including 54 from common CHB patients, 19 from patients with acute-on-chronic liver failure （HBV-ACLF）, and 20 from healthy controls by immunohistochemical staining. Semi-quantitative of the integral absorbance were conducted by the software Image-Pro-Plus 6.0. The correlation between the AIM-2 levels and the degree of liver damage were analyzed, respectively. Results AIM-2 expression was exclusive to the hepatic cellular cytoplasm in the liver tissue of patients with HBV. The median integral absorbance of AIM-2 in CHB patients was 4 996.88, which was significantly higher than that in healthy controls of 2296.49 （Z =--3.028, P = 0.002）, but was less than that in HBV-ACLF patients with 19 772.48 （Z =--5.008, P 〈 0.001）. The integral absorbance of AIM-2 in CHB patients was also positive correlated with the score of histological activity index （HAl） in liver （r = 0.707, P 〈 0.0001）. Conclusions AIM-2 rarely expressed in healthy liver, but significantly up-regulation were found in patients with chronic hepatitis B especially in patients with acute- on-chronic liver failure, indicating that AIM-2 may contribute to the pathogeneses of liver damage in chronic hepatitis B and liver failure.

关 键 词：黑色素瘤缺乏因子2 病毒性肝炎 乙型 慢性 肝脏组织 炎症反应 

分 类 号：R512.62[医药卫生—内科学]