CD1d^(hi)CD5^+CD19^+B细胞在HIV-1感染者外周血中的表达  

Expression of CD1d^(hi)CD5^+CD19^+B cells in peripheral blood from HIV-1 infected individuals

作　　者：党便利 李媛[1] 赵柯[1] 于卓然[1] 刘青[1] 郝春秋[1] 谢玉梅[1] 李新红[1] 康文臻[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,西安710038

出　　处：《中国艾滋病性病》2015年第6期457-460,469,共5页Chinese Journal of Aids & STD

基　　金：国家自然科学基金资助项目(81373144);国家科技重大专项课题资助项目(2012ZX09303013)~~

摘　　要：目的研究艾滋病病毒1型(HIV-1)感染者外周血中调节性B细胞(Breg)与T淋巴细胞数量、病毒载量及白介素-10(IL-10)的表达水平,分析其相关性并探讨Breg在HIV发病机制中的作用。方法选择30例未经抗病毒治疗的HIV-1感染者,分离外周血单个核细胞(PBMC),用流式细胞术(FCM)检测CD1dhi CD5+CD19+B细胞的比例、CD+4T淋巴细胞(简称CD4细胞)数量、CD+8T淋巴细胞(简称CD8细胞)数量,用酶联免疫吸附试验法(ELISA)测定其PBMC培养液上清中IL-10的表达水平,经反转录聚合酶链反应(RT-PCR)检测病毒载量,与同期选择的健康对照组比较。结果与健康对照组相比,HIV-1感染者的CD1dhi CD5+CD19+B细胞的比例均明显升高(P<0.0001),其培养液上清中IL-10的表达水平也显著升高(P=0.0005)。另外,HIV-1感染者PBMC中的CD1dhi CD5+CD19+B细胞的比例与CD4细胞数量(r=-0.6084,P=0.0004)及CD4细胞/CD8细胞的比值(r=-0.8715,P<0.0001)均呈显著负相关性,与血浆中病毒载量的水平(r=0.6825,P<0.0001)及IL-10的水平均呈正相关性(r=0.7656,P<0.0001),而与CD8细胞数量未见相关性(r=0.1183,P=0.5334)。结论CD1dhi CD5+CD19+B细胞比例与其相关细胞因子IL-10表达水平的上调可能参与HIV的发病机制。Objective To study the expression of Breg subsets, T lymphocyte cell counts, viral load and the lev el of IL-10 in peripheral blood from Chinese HIV-1 infected individuals,and analyze their correlation and explore the role of CD1db^hlCD^5＋ CD^19＋ B subsets in the pathogenesis of HIV. Methods The proportion of CD1db^hlCD^5＋ CD^19＋ B subsets,CD[ T cell counts and CD＋ T cell counts in peripheral blood were determined by FCM, the level of IL-10 in the supernatant of culture medium of PBMC was measured by ELISA and the level of viral load in the plasma was detected by RT-PCR in 30 patients with HIV and healthy controls. Result Compared to normal controls, the pro- portions of CD1db^hlCD^5＋ CD^19＋ B subsets were significantly increased （P〈0. 0001）, meanwhile the expression level of IL-10 in the supernatant of culture medium of PBMC of untreated group was higher than those in healthy controls （P= 0. 0005）. In addition, the proportions of CD1db^hlCD^5＋ CD^19＋ g subsets were inversely correlated with CD/T cell counts （r= -0. 6084,P=0. 0004）and CD4/CDs ratio （r= -0. 8715,P〈0. 0001）, positively correlated with the level of viral load （r=0. 6825,P〈0. 0001）and IL-10 （r=0. 7656,P〈0. 0001）, but not correlated with CD＋T cell counts （r= 0. 1183 ,P=0. 5334）. Conclusion The expression of CD1db^hlCD^5＋ CD^19＋ B subsets and IL-10 were in- creased due to HIV infection,which may correlate with the pathogenesis in HIV-1 patients.

关 键 词：艾滋病病毒1型感染者 调节性B细胞 发病机制 白细胞介素-10 

分 类 号：R512.91[医药卫生—内科学] K373.9[医药卫生—临床医学]