低磷胁迫对转基因抗虫玉米苗期Bt蛋白和磷利用效率的影响  

Effect of low phosphorus stress on Bt protein and P efficiencies in transgenic maize

作　　者：申婷婷[1] 李金[1] 苏贺[1] 詹延廷 蒲俊[1] 董学会[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2015年第3期15-23,共9页Journal of China Agricultural University

基　　金：农业部新品种培育重大专项(2013ZX08011)

摘　　要：为研究低磷胁迫（LP）、正常条件（HP）2个处理对转Bt基因玉米和郑单958根系形态以及磷利用效率的影响。以转Bt基因玉米（Bt799）和受体郑单958为试验材料,进行温室水培试验。研究证明：1）低磷胁迫后Bt799的根长、根鲜重和根冠比升高幅度高于郑单958,就总根长、根系投影面积总和、根表面积、根平均直径、单位立方米总根长和根总容积而言,Bt799玉米的LP/HP是郑单958玉米LP/HP的1.13、1.19、1.33、1.18、1.14和1.55倍。2）低磷胁迫降低各叶位Bt蛋白量,根部除根基处1～7cm Bt蛋白量上升以外,其余根系均下降。在正常处理下,Bt799全株和根系Bt蛋白总量为1 750和423ng,低磷处理下Bt799分别为1 470和784ng。3）低磷胁迫下2种玉米叶片和根系磷含量和磷积累量显著降低,各叶位磷占总磷比例显著下降,但根系磷占总磷比例反而升高。研究表明,Bt799通过根长、根表面积和根系直径等形态变化来优化其空间分布构型,吸收更多的磷元素,但向叶片转运磷元素的能力较弱。To clarify the effects of low-phosphorous on Bt protein, root morphology and efficiencies,we took Bt799 and the back-ground 7hengDan958 as experimental materials to carry out hydroponics test. The results showed that： 1）Root length,root fresh, weight and root shoot ratio of Bt 799 rose more than those of ZhengDan958 under P-deficient condition. The ratio of P-dificient and P-sufficient （LP/HP） of Bt 799 were 1.13,1.19,1.33,1.18,1.14 and 1.55 times higher than LP/HP （Zhengdan 958） in root total length, root projected area, root surface area, root average diameter, root total length per cubic meter and root volume. 2）The leaf Bt protein expression content decreased, the same as root except 1-7 cm root base. Total Bt protein content of entire Bt799 and root were 1 750 and 423 ng under P-sufficient, while under P-deficient condition they were 1 470 and 784 ng. 3） In different positions of both maize, P concentration and P accumulation decreased dramatically under P-deficient, the percentage of leaf P amount to total P amount decreased significantly, root showed the opposite trend. We conclude that Bt 799 optimized the spatial distribution of root to absorb more P element through the changes of morphological characteristics such as root length, root surface area, root diameter and so on,but the P transport ability to upper leaf is weak.

关 键 词：低磷胁迫 转BT基因玉米 BT蛋白 根系形态 磷利用效率 

分 类 号：S513[农业科学—作物学]