木麻黄(Casuarina equisetifolia)内生真菌的分离与促生菌株的筛选  

Isolation of Endophytic Fungi from Casuarina equisetifolia and Screening of Promoting Strains

作　　者：林燕青[1,2] 吴承祯[2,3] 谢安强[1,2] 林晗[1,2] 李键[1,2] 洪滔[1,2] 洪伟[1,2] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省高校森林生态系统过程与经营重点实验室,福建福州350002 [3]武夷学院生态与资源工程学院,福建南平354300

出　　处：《北华大学学报（自然科学版）》2015年第4期522-528,共7页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金项目(31400533);福建省自然科学基金项目(2014J01074)

摘　　要：采用组织分离法从福建省大鹤林场不同年龄木麻黄各组织器官中分离与纯化得到65株内生真菌.研究木麻黄内生真菌在其器官构件内的分布规律,同时按水培和盆栽两种方式侵染无性系苗,测定接菌苗木的新梢长度,筛选出促生长的菌株.结果表明:木麻黄内生真菌主要分布于植株的鳞状叶小枝和根中,分别占筛选出菌株数量的40.0%和41.5%,且随着树龄的增大,内生真菌的数量减少;在水培苗浸泡菌液方式下的接菌处理能显著增加苗木的平均抽梢量,促进水培苗生长.其中,菌株18处理下苗木的平均抽梢量为54.85 cm,是对照苗木的7.62倍.The present investigation was undertaken in order to isolate endophytic fungi from Casuarina equisetifolia by using the tissue separating method and then study the distribution of endophytic fungi under the different forest-stands and organs. This paper aimed to screen out the most beneficial strains so as to promote the growth of water cultured Casuarina seedling. 65 different endophytic fungi strains were isolated,purified and then inoculated to Casuarina clones seedlings by using two different inoculation methods which are dipping the seedling root in suspension （ S ） and irrigating the potted seedling with suspension （ P ） . The results showed that these strains were mainly distributed in scale-like leaves and roots,accounting for 40% and 41. 5% of all the screened strains respectively, furthermore, with the increasing tree-age, the amount of endophytic fungi will reduce. Most strains screened out from Casuarina equisetifolia can promote the quantity of shoots growth,moreover the S method is superior to the P method in this study. In the case of using S method,the average quantity of shoots growth of strain No. 18 is the maximum,which was up to 54. 85 centimeters and it was 7. 62 times as much as CK.

关 键 词：木麻黄 内生真菌 分离 促生菌株 

分 类 号：S718.81[农业科学—林学]