毛细管电泳法研究牛血清白蛋白与盐酸异丙肾上腺素的相互作用  

Studies on the Interaction Between Bovine Serum Albumin and Hydrochloric Acid Isoproterenol by Capillary Electrophoresis

作　　者：刘春叶[1] 张雪娇[1] 苗延青[1] 赵灵芝[1] 赵敏[1] 

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《分析科学学报》2015年第3期313-317,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.81202492;21305109);陕西省科技厅项目(No.2014K02-11-01;2014JQ2073);陕西省大学生创新创业实践项目训练计划(No.201211840003);西安医学院校基金(No.12FZ10)

摘　　要：本研究分别采用区段-区段动力学毛细管电泳法(Plug-Plug Kinetic Capillary Electrophoresis,ppKCE),及以药物为添加剂的亲和毛细管电泳法(Affinity Capillary Electrophoresis,ACE)对盐酸异丙肾上腺素(Hydrochloric Acid Isoproterenol,HAI)与牛血清白蛋白(Bovine Serum Albumin,BSA)的结合作用进行研究。实验结果显示,ppKCE法可同时测得HAI与BSA相互作用体系的结合速率常数kon和离解速率常数koff分别为163.60L·mol-1·s-1、3.50×10-2±0.95×10-2s-1(n=3)。ppKCE法和ACE法测得HAI与BSA的结合常数Kb分别为4.67×103 L·mol-1、6.02×103 L·mol-1,两种方法所得结果能够较好吻合。证明毛细管电泳法在药物-蛋白相互作用研究领域的可靠性,可用于测定药物与蛋白的相互作用,从而用于新药筛选研究。The interaction of hydrochloric acid isoproterenol（HAI）and bovine serum albumin（BSA）was investigated by capillary electrophoresis（CE）.The rate constants of complex formation kon and the dissociation koffbetween HAI-BSA interaction were detected by plug-plug kinetic capillary electrophoresis（ppKCE）,which were 163.60L·mol^-1·s^-1 and 3.50×10^-2±0.95×10^-2s^-1（n=3）,respectively.The binding constant Kb of HAI-BSA detected by ppKCE was 4.67×10^3 L·mol^-1.The Kb obtained by affinity capillary electrophoresis（ACE）was 6.02×10^3 L·mol^-1 with drug and protein as additives.The results indicated that the two methods matched well with each other.It indicates that CE is reliable and available in drug-protein interaction research.The bind constant of drug-protein can be obtained by CE,which is useful for drug screening.

关 键 词：区段-区段动力学毛细管电泳法 亲和毛细管电泳 结合常数 

分 类 号：O657.8[理学—分析化学]