APX基因植物表达双元载体的构建及转化剑麻  

Construction of APX Gene Plant Binary Expression Vector and Transformation of Sisal

作　　者：覃海燕[1] 徐洪伟[1] 高建明[2] 鹿志伟 莫廷辉[1] 易克贤[3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《热带作物学报》2015年第6期1112-1118,共7页Chinese Journal of Tropical Crops

基　　金：利用转基因技术创制抗斑马纹病剑麻新种质的研究(No.31371679)

摘　　要：剑麻是中国热带地区最重要的麻类经济作物。剑麻H.11648是中国唯一的当家品种,此品种虽高产但易感斑马纹病,而现有的化学农药防效差且病原菌易产生抗性。为了培育出剑麻抗病新品种,以Pcambia3300为基础质粒,构建由35S驱动的APX基因的植物表达载体Pcambia3300-35S-APX-nos,再通过农杆菌EHA105将其导入剑麻中,经PCR扩增后电泳检测分析,结果表明外源基因已整合到受体植物基因组中。Sisal is one of the most important cash crops in the tropical areas of China. Sisal H. 11648 is China＇s only variety dorminantly planted. The variety is of high yield but susceptable to zebra grain, and the control effect of chemical pesticides is poor and the pathogens are prone to resistance. In order to breed new sisal disease- resistant varieties, Agrobacterium-mediated APX gene transformation of sisal was tried. On the basis of Pcambia 3300 plasmid, an APX＇S plant expression vector Pcambia-3300-APX which driven by 35S was built and transformed to sisal by agrobacterium EHA105. PCR amplification and electrophoresis detection analysis showed that the exogenous gene was integrated into the receptor plant genome.

关 键 词：剑麻 APX基因 斑马纹病 转化 

分 类 号：S563.8[农业科学—作物学]