四倍体金银花水提物抗炎作用和急性毒性实验研究  

Experimental study on anti-inflammatory effects and acute toxicity of aqueous extract from tetraploid Lonicerae Flos

作　　者：胡璇[1,2] 李卫东[2,3] 张硕峰[2] 李欧[2] 郝江波[2,3] 莫愁[2,3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]北京中医药大学中药学院,北京100102 [3]北京中医药大学中药材规范化生产教育部工程研究中心,北京100102

出　　处：《中草药》2015年第11期1649-1652,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31370360)

摘　　要：目的对四倍体金银花水提物的抗炎作用以及急性毒性进行评价。方法以二倍体金银花为对照,分别采用角叉菜胶诱导大鼠足肿胀实验、大鼠棉球肉芽肿实验对四倍体金银花水提物进行抗炎活性评价;采用Bliss法测定小鼠的半数致死量(LD50)对其进行急性毒性评价。结果四倍体金银花水提物具有明显的抗急性炎症作用,与亲本二倍体金银花之间抗炎作用差异不具有统计学意义,2个种质均不具有明显的抗亚急性炎症作用。四倍体和二倍体金银花水提物ig给药对小鼠的LD50值分别为72.12、69.92 g/kg,急性毒性差异不明显,分别相当于人体(体质量60 kg)安全用量的412和400倍。结论四倍体金银花水提物具有明显的抗急性炎症作用,在一定剂量范围内使用较为安全。Objective To evaluate the anti-inflammatory effects and acute toxicity of the aqueous extract form tetraploid Lonicerae Flos. Methods Compared with the diploid, the paw swelling of rats induced by carrageenan and cotton pellet granuloma tests were obtained to study the anti-inflammatory effects of the tetraploid Lonicerae Flos. A pretest was used to judge the possibility of LD50 in the acute toxicity test. The experimental data are calculated LD50 on mice by Bliss method to evaluate the acute toxicity of the diploid Lonicerae Flos. Results The tetraploid Lonicerae Flos had the significant resistance to acute inflammation, but there was not significance between the two ploidies. Neither the tetraploid nor the diploid had the obvious subacute inflammation resistance effects. The possibility of measuring LD50 was definite through pre-test. The LD50 of the aqueous extract from the two ploidies were 72.12 and 69.92 g/kg, respectively. However, there was no obvious difference in acute toxicity between the two ploidies. And the LD50 of mice was equal to 412 and 400 times of 60 kg normal human＇s daily dried medicinal herb expenses respectively. Conclusion The tetraploid Lonicerae Flos has the significant anti-inflammation with less toxic and side effect. It could be used safely in a certain dose range.

关 键 词：金银花 四倍体 二倍体 抗炎作用 急性毒性 

分 类 号：R285.5[医药卫生—中药学]